Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Letters in Biotechnology》 2007-05
Add to Favorite Get Latest Update

The Establishment of Extraction Method of Taxol from Taxus brevifolia

WANG Xiu-jun,ZHANG Wei,WANG Jun Forestry College,Northeast Forestry University,Harbin 150040,China  
Objective:To eatablish an effect,fast method of extraction and examine of taxol from Taxus brevifolia,some factors such as granularity of comminution,extractant,extraction time,the rate of solution to solid were studied.Methods:With leaf of T.brevifolia as raw material,the organic impregnant-ultrasonic extract method was used.The detection and quantitative analysis were carried on in HPLC method and standard curve method respectively.Results:The optimum conditions of extraction were as follows,the material was desiccation to constant weight at 95℃,and extracted with 95% carbinol at 60℃ for 20 h,the rate of solution to solid 1:10.Ratio of extraction was 0.086 mg/g.Conclusion:The method is brief,fast,nicety and better adjustability,which supplied a good tool for the extraction and detection of taxol.
【Fund】: 中国野生植物保护协会科研项目(010-413255)
【CateGory Index】: Q946
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 JIN Zhen-lan et al(College of Agronomy,Yanbian University,Longjing,Jilin 133400);Effects of Different Medium on Callus Induction from Taxus cuspidate Sied. et Zucc.[J];Journal of Anhui Agricultural Sciences;2010-19
2 LIU Yan-ze1,2,GAO Wen-qin3,WANG Ji-wen4,ZHANG Yu3,ZHAO Yu-qing4,5 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China 2.Bio-Organic and Natural Product Laboratory,McLean Hospital/Harvard Medical School,MA 02478,USA 3.Shenyang Pharmaceutical University,Shenyang 110006,China 4.Shenyang Tianfeng Bioengineer Technology Co.,Ltd.,Shenyang 110015,China 5.Key Laboratory of Structure-Based Drug Design & Discovery,Ministry of Education,Shenyang Pharmaceutical University,Shenyang 110016,China;Smashing Tissue Extraction and HPLC Determination of Paclitaxel and 10-Deacetylbaccatin from Taxus x media[J];中草药(英文版);2011-03
3 Wang Haiying(College of Forestry,Northeast Forestry University,Harbin 150040,China);Teaching Practice and Expansion in Medicinal Plant Resources[J];Guangdong Chemical Industry;2012-09
4 Mou Lihui;Cheng Yishao;Zheng Qinsheng;Life Sciences Academy,Jiaying University;;Extraction and Determination of Paclitaxel Content in the Cortices of Amentotaxus Angotaenia(Hance) Pilger[J];Guangdong Chemical Industry;2014-04
5 LI Jian,LIU Yu,SHI Mei-hui,TANG Qing,WU Zhuo-na,DOU Gui-fang,MENG Zhi-yun (Institute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing 100850,China);Determination of paclitaxel content in different parts of Taxus wallichiana var. mairei by HPLC internal standard method[J];Military Medical Sciences;2011-08
6 GAO Shu-yun,CHEN Yan,DU Xi-hua(School of Chemistry and Chemical Engineering,Xuzhou Institute of Technology,Xuzhou 221008,China);Extraction of Taxol from Taxus cuspidate with Cell Disrupture Backflous Methed[J];Lishizhen Medicine and Materia Medica Research;2010-12
7 Yu Xianghua;Dai Yongqiang;Shao Jinhua;Department of Life Science & Chemistry Engineering,Hunan Science & Technology College;;Study on Ethanol Extracting Conditions of Taxol from Leaves of Taxus Chinese var. maria[J];Biotechnology Bulletin;2013-10
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Chun-bin TONG Jing-jing FAN Sheng-di (College of Life Science, Dalian Nationalities University, Dalian Liaoning 116600,China);Improved Purification Determination of Taxol[J];Journal of Dalian Nationalities University;2005-05
2 Zheng Guangle, Ge Binbin, Huang Tianwei, Fu Ruishu ( Fujian Institute of Testing Technology, Fuzhou, Fujian, 350003; Department of Pharmacology, Fujian College of Traditional Chinese Medicine, Fuzhou, Fujian, 350003; Sanming Forestry Bureau, Sanming, Fujian, 365000));HPLC method to analyze taxol in taxus[J];Fujian Analysis&Testirg;2004-Z1
3 WU Qian,GAO Qing-ping,WANG Zheng-ping(Chemical Engineering College,Harbin Engineering University, Harbin 150001,China);Study on extraction and pretreatment methods of taxol[J];Chemical Engineer;2004-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIN Zhen-lan et al(College of Agronomy,Yanbian University,Longjing,Jilin 133400);Effects of Different Medium on Callus Induction from Taxus cuspidate Sied. et Zucc.[J];Journal of Anhui Agricultural Sciences;2010-19
2 QIU Sheng-rong, ZHU Chao-zhi (Fujian Agriculture and Forestry University Jinshan College,Fuzhou 350002,China );How to Develop the Yew Industry in the County of Mingxi[J];Science Technology and Industry;2009-06
3 LI Chun-bin TONG Jing-jing FAN Sheng-di (College of Life Science, Dalian Nationalities University, Dalian Liaoning 116600,China);Improved Purification Determination of Taxol[J];Journal of Dalian Nationalities University;2005-05
4 SUN Xiao-jun,ZHANG Zong-shenSchool of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China;Preparation of artifical seeds of Taxus cuspidata CV. Nana[J];Journal of Dalian Polytechnic University;2010-05
5 YANG Xian1,2,YANG Shui-ping1,ZHANG Xue1,TAN Jie2 (1College of Resource and Environment,Southwest University,Chongqing 400716; 2Chongqing Sino Biology Pharmaceutical Co. LTD.,Chongqing 410041,China);Optimization of taxol extraction from Taxus baccata L.[J];Acta Academiae Medicinae Militaris Tertiae;2007-19
6 FU Ruo-nong(Department of Chemistry,Beijing Institute of Technoligy,Beijing 100081);Advances on SPE-chromatography in China in recent two years[J];Chinese Journal of Analysis Laboratory;2007-02
7 LI Feng-tao,RONG Jun-dong,CHEN Li-guang,GUO Xi-jun,ZHANG Ling-ling,ZHENG Yu-shan(Institute of Industrial Forest,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Advances in Taxus spp. based on GAP[J];Subtropical Agriculture Research;2008-02
8 Qin Zhuli Jiang Yuanru(School of Natural Science,Xi'an University of Architecture and Technology,Shanxi Xi'an 710055);Applications of Supercrifical Fluid Exiraction Technique in Alkaloids Extraction[J];Chemical Industry Times;2006-07
9 MAN Rui-lin,QIAO Liang-jie,NI Wang-dong(School of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China);The research of extracting taxol from the branches of taxus media with the method of ultrasonic[J];Chemical Research and Application;2008-12
10 ZHANG Ran1,2,SUN Rui1,2,FU Yu-jie1,2,LI Shuang-ming1,2,GU Cheng-bo1,2,LIU Wei1,2,LUO Hao1,2,ZHAO Chun-jian1,2(1.Key Laboratory of Forest Plant Ecology of the Ministry of Education;2.Engineering Research Center of Forest Bio-preparation of the Ministry of Education,Northeast Forestry University,Harbin 150040,Heilongjiang Province,China);Pre-separation process for extraction solutions of paclitaxel with methanol-water system[J];Chemical Engineering(China);2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Wen-jie et al(Department of Life Science,Hengshui University,Hengshui,Hebei 053000);Research on Biological Characteristics of Taxus media[J];Journal of Anhui Agricultural Sciences;2007-08
2 YIN Yong (Anhui Provincial Center for Forestry High Technology Development,Hefei,Anhui 230031);Contrast Study on the Survival Rate of the Cuttings of Taxus media[J];Journal of Anhui Agricultural Sciences;2008-22
3 CAI Yu-mei et al (Dujiangyan College of Sichuan Agriculture University,Dujiangyan,Sichuan 611830);Determination of Taxol Content in Root of Taxus media in Different Ages by HPLC[J];Journal of Anhui Agricultural Sciences;2010-28
4 WANG Dong-mei et al(College of Forestry,Northwest A &F University,Yangling,Shaanxi 712100);Teaching Reform on Natural Products Chemistry of Forestry Chemical Engineering in Agriculture and Forestry University[J];Journal of Anhui Agricultural Sciences;2011-32
5 WANG Tingqin1,YAO Huaxiong2,LI Zhongming1(1.College of Agriculture,Guangdong Ocean University,Zhanjiang,Guangdong 524088;2.Beijing Xinhefeng Agrochemical Co.,Ltd.);Effect of Manganese on Growth and Quality of Amaranth[J];Journal of Changjiang Vegetables;2011-04
6 TANG Qian1,YANG Yuan-juan1,YANG Xian2 (1.Chongqing Medical and Pharmaceutical College,Chongqing 400030,China;2.Chongqing Sino Pharmaceutical Co.,Ltd.,Chongqing 400041,China);Process optimization of supercritical fluid extraction of active ingredients in taxus[J];Chongqing Medicine;2010-23
7 YI Xia1,LI Yun-qi1,AOTEGEN·Bai-yin1,XU Min-yun1,ZHANG Lan-lan1,ZHANG Yong-jiang2(1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China;2.Qiaodong Distric Agricultural Barean of Xingtai city,Xingtai 054001,China);Effects of five trace element mixed applications on hay yield of alfalfa[J];Journal of Agricultural University of Hebei;2009-03
8 LIU Tie yan,LIU Yun,ZHAO Cai feng,ZHENG Yi zhi (School of Life Science,Northeast Normal University,Changchun 130024,China);Study on callus induction and tissue culture of Taxus cuspidata[J];Journal of Northeast Normal University (Natural Science Edition);2002-02
9 LI Chun-bin TONG Jing-jing FAN Sheng-di (College of Life Science, Dalian Nationalities University, Dalian Liaoning 116600,China);Improved Purification Determination of Taxol[J];Journal of Dalian Nationalities University;2005-05
10 YANG Xian1,2,YANG Shui-ping1,ZHANG Xue1,TAN Jie2 (1College of Resource and Environment,Southwest University,Chongqing 400716; 2Chongqing Sino Biology Pharmaceutical Co. LTD.,Chongqing 410041,China);Optimization of taxol extraction from Taxus baccata L.[J];Acta Academiae Medicinae Militaris Tertiae;2007-19
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 KONG De-xin1,HUANG Xi-yang1*,LI Feng1,3,JIANG Shui-yuan1,HUANG Shu-shi2,LI Hong11.Guangxi Institute of Botany,Chinaese Academy of Sciences,Guilin 541006,Chinaa2.Lab of Biophysics,Guangxi Academy of Sciences,Nanning 530003,Chinaa3.Guangxi Academy of Sciences,Nanning 530003,China;Analysis and Evaluation of Taxol Contents in Different Parts between Taxus media and Taxus mairei Based on Fourier Transform Infrared Spectroscopy[J];Spectroscopy and Spectral Analysis;2011-03
2 JIN Ling-ling et al(Agriculture College,Yanbian University,Yanbian,Jilin 133002);Study on Rooting in vitro of Phellodendron amurense[J];Journal of Anhui Agricultural Sciences;2013-04
3 TANG Yun 1 , LIU Yan-ze 2, 3 , HAN Ling 4 , ZHAO Yu-qing 1, 5 1. Shenyang Pharmaceutical University, Shenyang 110016, China 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China 3. Bio-organic and Natural Product Laboratory, McLean Hospital/Harvard Medical School, MA 02478, USA 4. Liaoning Benxi Third Pharmaceuticals Co., Ltd., Benxi 117004, China 5. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China;Optimization of Smashing Tissue Extraction Technology of Schisandra chinensis Fruits by Orthogonal Test[J];中草药(英文版);2012-03
4 TANG Wei-zhuo1, LIU Yan-ze2, 3, ZHAO Yu-qing1, 4* 1. Shenyang Pharmaceutical University, Shenyang 110016, China 2. Bio-Organic and Natural Product Laboratory, MRC 311, McLean Hospital/Harvard Medical School, MA 02478, USA 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193 4. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China;A New Homogenizing Technology to Obtain Rosmarinic Acid from Perilla Oil Meal[J];中草药(英文版);2012-01
5 WANG Chao, HOU Yi-chong, WANG Wen, WANG Guo-fu Department of Life Science, Yuanpei College, Shaoxing University, Shaoxing, Zhejiang 312000, China;Effect of Winter LED Supplementary Illumination on Growing Development of Taxus chinensis var. mairei Cheng et L.K[J];药用植物研究(英文版);2012-10
6 CHEN Jian-feng,LI Ke-ke,WANG Cheng-en,DENG Tian,DONG Shi-chao,YANG Miao-xian(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China);Preliminary Report on in vitro Culture of Nothapodytes nimmoniana Stem Segment[J];Hubei Agricultural Sciences;2013-07
7 Ma Ying Li Hongzhu Yang Kai(First-author's address: Forestry Research Institute of Heilongjiang Province,Harbin 150081,Heilongjiang,China);Talk About the Research Progress of the Propagation Technique of Taxus cuspidate Sieb.et Zucc[J];Forest Investigation Design;2013-01
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 XIAO Guoyong1,LIN Bingchang1,MA Zidu2(1.Center of Seperation Technology, Anshan Institute of I. & S. Technology,Anshan 114002,China;2.University of Purdue,Ind U.S.A.);The Analysis of Extracting Process of Taxol fromthe Northeastern Taxus Leaves[J];JOURNAL OF ANSHAN INSTITUTE OF IRON AND STEEL TECHNOLOGY;1999-01
2 Bao Yihong Wang Zhenyu (Department of Food Science and Engineering, Northeast Forestry University, Harbin 150040);A Survey of Taxol Research and the Trends[J];Quarterly of Forest By-product and Speciality In China;2003-02
3 Zheng Guangle, Ge Binbin, Huang Tianwei, Fu Ruishu ( Fujian Institute of Testing Technology, Fuzhou, Fujian, 350003; Department of Pharmacology, Fujian College of Traditional Chinese Medicine, Fuzhou, Fujian, 350003; Sanming Forestry Bureau, Sanming, Fujian, 365000));HPLC method to analyze taxol in taxus[J];Fujian Analysis&Testirg;2004-Z1
4 WU Qian,GAO Qing-ping,WANG Zheng-ping(Chemical Engineering College,Harbin Engineering University, Harbin 150001,China);Study on extraction and pretreatment methods of taxol[J];Chemical Engineer;2004-11
5 Liu Pei, Jiao Yubihg, Lui Chuanyu,Xu Junmei,Suen Chao, and Kang Jianhua(Institute of Petrochemistry,Heilongjiang Academy of Sciences, Harbin 150050);Development of Taxol—the New antitumer Agent[J];CHEMISTRY AND ADHESION;1998-03
6 Yuan Yingjin; Na Ping; Hu Zongding(Dept. of Chem. Eng. Tianjin University, Tianjin 300072)Wang Daobin; He Binglin(Inst. Polymer Chem., Nankai University, Tianjin 300071);Primary Study on Decoloration of Taxus Cuspidata Extract by Adsorption[J];ION EXCHANGE AND ADSORPTION;1994-06
7 Wang Aimin(Analysis and Test Center,Guizhou Normal University,Guiyang,550001);DETERMINATION OF CONTENT OF TAXOL BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY[J];JOURNAL OF GUIZHOU NORMAL UNIVERSITY(NATURAL SCIENCE);1998-02
8 ZHU Shun-qin 1, TAN Feng 1, TANG Ke-xuan 2 (1.Institute of Modern Biopharmaceuticals,Department of Life Science,Southwest China Normal University,Chongqing 400715,China; 2. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China);Technology progress on extraction and purification of taxol[J];Chinese Journal of Biochemical Pharmaceutics;2004-01
9 Mu Dehai, Zhou Zhifa, Lin Zhanjiang, Li Guangxian, Liu Jianzhong, Li Jiansheng (Chinese National Analytical Center, Guangzhou, Guangzhou 510070);Study on Analysis of Taxol and Its Analogues in Taxus by Reversed Phase High Performance Liquid Chromatography[J];JOURNAL OF INSTRUMENTAL ANALYSIS;1997-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved