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《Letters in Biotechnology》 2011-03
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Effects of IFN-α2a-NGR Combined with 5-Fluorouracil on the Sensitivity of A549 Cell Line

SUI Wen-Jun1,2,LIU Yong-Lan1,2,LEI Xiao-Ying1,2,HUANG Qi-Chao1,2, GUO Yan-Hai1,2,WANG Wei1,2,ZHANG Ying-Qi1,2,YAN Zhen1,2 1.State Key Laboratory of Tumor Biology;2.School of Pharmacy,the Fourth Military Medical University; Xi'an 710032,China  
Objective:To observe the effects of IFN-α2a-NGR,5-fluorouracil(5-FU) and their combination on the proliferation and apoptosis of A549 cell line.Methods:IFN-α2a-NGR(500 U/mL) and 5-FU(1.0 μg/mL) were used.A549 cells were divided into control group,IFN-α2a-NGR treated group,5-FU treated group,IFN-α2a-NGR+5-FU treated group.The inhibition on the proliferation of A549 cells respectively treated with IFN-α2a-NGR,5-FU and their combination was detected by MTT,and their apoptosis rate was detected by FCM,cell morphology was observed by fluorescence microscropy.Results:The inhibition rate and apoptosis rate of IFN-α2a-NGR+5-FU treated cells significantly higher than those of IFN-α2a-NGR alone treated cells and 5-FU alone treated cells.Conclusion:IFN-α2a-NGR combined with 5-FU can synergistically induce the inhibition and apoptosis of A549 cell line.
【Fund】: 国家自然科学基金(30572210);; 陕西省自然科学基金(2009JM4005);; 西安市科技计划(SF09027)
【CateGory Index】: R734.2
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 MENG Jie-Ru, YAN Zhen, ZHAO Ning, ZHANG Ying-QiBiotechnology Centre, Department of Pharmaceutics, Fourth Military Medical University, Xi’an 710033, China;High cell density fermentation of engneering E. coli expressing tumor targeted interferon-α2a[J];Journal of The Fourth Military Medical University;2004-23
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【Co-citations】
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1 ZHANG Chun, CONG Yan-guang, HU Fu-quan, RAO Xian-cai (Department of Microbiology, College of Medicine, Third Military Medical University, Chongqing 400038, China);Reconstruction of engineered bacteria in preparing recombinant human peptide antibiotic β[J];Acta Academiae Medicinae Militaris Tertiae;2006-22
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7 XUE Xiao-chang, LI Meng, QIN Xin, et al (Biotechnology Center, Department of Pharmacy, Fourth Military Medical University, Xi′an 710032, China);Stability of Biological Characteristics of Recombinant E. coli Expressing Human B Lymphocyte Stimulator[J];Chinese Journal of Biologicals;2008-09
8 REN Bing-xiu~1 WANG Jing~1 ZHANG Lu~2 WANG Zhe~1 YANG Wei-dong~1 ZHANG Ying-qi~2 LI Guo-quan~1 HE Li-qing~2(1.Department of Nuclear Medicine,Xijing Hospital,the Forth Military Medical University of PLA,Xi'an 710032,China;2.Center of Biotechnology,the Forth Military Medical University of PLA,Xi'an 710032,China);Study of Indirect ~(99)Tc~m Labelling NGR-IFN-α2a[J];Journal of Isotopes;2008-04
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【Secondary Citations】
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