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《Letters in Biotechnology》 2011-03
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Establishing on Genetic Regeneration System of Maize Inbred Lines Dan598

CHEN Lan,WU Ying,LIU Hong-Kui,YUAN Ya-Ping Plant Science College of Jilin University,Changchun 130062,China  
Objective:To induce callus from immature embryos of maize inbred lines Dan598 for establishment of regeneration system.Methods:The effects of embryo age,media type,concentration of 2,4-D on the callus induction were studied.Results:It was indicated that 16~18 days after post-pollination,2,4-D concentration at 2.0 mg/L are the best condition to induct embryogenic callus;set 5 kinds of culture medium such as N6,improved NB,NB,MS,MB,to observe the effects of callus induction,select the culture medium of induction with improved NB medium is better than other mediums.Differentiation medium suppled with 1 mg/L KT,0.5 mg/L 6-BA and 0.5 mg/L NAA can promote the shoot differentiation and root growth.Conclusion:An efficient plant regeneration system has been established for maize inbred lines Dan598,which provides basis for further genetic transformation.
【Fund】: 国家转基因生物新品种培育重大专项(2009ZX08003-024B)
【CateGory Index】: S513
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