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《Letters in Biotechnology》 2013-04
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Optimization of L-Glutamine Fermentation Medium by Response Surface Method

GUO Chun-Qian1,JIANG Yan-Zhe1,GUAN Dan2,ZHANG Da-Long1,XU Qing-Yang1*,XIE Xi-Xian1,ZHANG Cheng-Lin1,CHEN Ning1 1.Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Bioengineering,Tian jin University of Science and Technology,Tianjin 300457;2.China Biotech Fermentation Industry Association,Bei jing 100833;China  
Objective:Response surface method was applied to optimize the fermentation medium components for improving L-glutamine production.Methods:The important factors influencing L-glutamine yield,which was identified by initial screening method of Plackett-Burman,were glucose,corn steep liquor and(NH4)2SO4.The path of steepest ascent was undertaken to approach the optimal region of the three significant factors.Central com posite design and response surface method were adopted to further investigate the mutual interaction between the variables and identify optimal values that bring maximum L-glutamine yield.Results:Experimental results showed that the optimal conditions for the yield were glucose 100 g/L,corn steep liquor 4.5 ml/L,(NH4)2SO4 37.2 g/L,and the maximum predicted value of L-glutamine yield was 41.0 g/L.Conclusion:After the checking experiment,the maximum predicted value was consistent with mean value of verification test and L-glutamine yield was in creased by 37.6% under optimized condition.
【CateGory Index】: TQ920.6
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