Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Letters in Biotechnology》 2013-04
Add to Favorite Get Latest Update

Optimization of L-Glutamine Fermentation Medium by Response Surface Method

GUO Chun-Qian1,JIANG Yan-Zhe1,GUAN Dan2,ZHANG Da-Long1,XU Qing-Yang1*,XIE Xi-Xian1,ZHANG Cheng-Lin1,CHEN Ning1 1.Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Bioengineering,Tian jin University of Science and Technology,Tianjin 300457;2.China Biotech Fermentation Industry Association,Bei jing 100833;China  
Objective:Response surface method was applied to optimize the fermentation medium components for improving L-glutamine production.Methods:The important factors influencing L-glutamine yield,which was identified by initial screening method of Plackett-Burman,were glucose,corn steep liquor and(NH4)2SO4.The path of steepest ascent was undertaken to approach the optimal region of the three significant factors.Central com posite design and response surface method were adopted to further investigate the mutual interaction between the variables and identify optimal values that bring maximum L-glutamine yield.Results:Experimental results showed that the optimal conditions for the yield were glucose 100 g/L,corn steep liquor 4.5 ml/L,(NH4)2SO4 37.2 g/L,and the maximum predicted value of L-glutamine yield was 41.0 g/L.Conclusion:After the checking experiment,the maximum predicted value was consistent with mean value of verification test and L-glutamine yield was in creased by 37.6% under optimized condition.
【CateGory Index】: TQ920.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 DING Bang-qin,QIU Xin,Zhou Feng(Nantong Vocational College,Chemical Engineering Department,Nantong 226007, China);Summary of L-Glutamine Biochemical Properties,Application and Production[J];Amino Acids & Biotic Resources;2008-04
2 WANG Shi hua 1, BAI Wen zhao 2,KANG Xu 1, WU Si fang 1 (1 Dep. of Biological Engin., Hubei Polytechnic Univ., Wuhan 430068, China; 2 Dep. of Biological,Hanzhong Teachers college,Hangzhong 723000, China);The Quantitative Determinations of L-Glutamine in Fermanted Liquor[J];Journal of Hubei Polytechnic University;2001-01
3 XIAO Huai-qiu and LI Yu-zhen (Applied Chemistry Department of Hu'nan Chemical Industry and Vocational Technology College, Zhuzhou,Hu'nan 412004, China);Research Progress in the Optimization of Microbial Culture Medium[J];Liquor-Making Science & Technology;2010-01
4 LIU Li-jun, ZHAO Jin (Chemical Engineering Institute,Tianjin University,Tianjin,300072,China);Advance in studing on L-glutamine[J];Tianjin Chemical Industry;2003-01
5 YANG Shi-Quan ZHANG Xi-Cheng LIU Jun-Feng WANG Fang TAN Tian-Wei DENG Li (College of Life Science and Technology,Beijing University of Chemical Technology,The Key Laboratory of Bioprocess of Beijing,The Biorefinery Research and Engineering Center of the Ministry of Education of China,Beijing 100029,China);Response Surface Methodology to Optimize Lipid Production Conditions by Saccharomyces cerevisiae[J];Microbiology China;2010-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DING Bang-qin et al(Department of Chemical Engineering,Nantong Vocational College,Nantong,Jiangsu 226007);Study on High-efficient Enzymatic Conversion Conditions of Glutamine[J];Journal of Anhui Agricultural Sciences;2009-18
2 YANG Chao LI ChunZhen TAN TianWei (College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Optimization of glucose oxidase production by fermentation with Aspergillus niger using a response surface method[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2011-01
3 GUO Wei,LI Qin-fan,KONG Xiang-ya,REN Jie,JING Xin-tang(College of veterinary medicine,Northwest A&F University,Yangling,Shaanxi Province 712100,China);Optimal C/N Ratio for Producing Swainsonine(SW) Using Locoweed Endophytes FEL3[J];Acta Agrestia Sinica;2011-04
4 Du Guangyinghui,Cui Daizong,Zhang Ning,Zhao Min,Li Guofang,Gu Xiaoxu(College of Life Sciences,Northeast Forestry University,Harbin 150040,P.R.China);Optimization of Culture Medium Composition for Azoreductase Production by Escherichia coli CD-2 Using Response Surface Methodology[J];Journal of Northeast Forestry University;2012-05
5 ZHANG Huan,CONG Li-na,HOU Ying-min,WANG Dan,XIE San-qun (School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China);Medium optimization for spore production of marine Bacillus subtilis HS-A38 by response surface methodology[J];Journal of Dalian Polytechnic University;2012-01
6 HONG Mei1 DIAO Qiyu1 YAN Guilong2 TU Yan1 ZHANG Naifeng1 JIANG Chenggang1(1.Key Laboratory of Feed Biotechnology of Ministry of Agriculture,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Hebei North University,Zhangjiakou 075000,China);Optimization of Culture Conditions for Silage Lactic Acid Bacteria via a Response Surface Technique[J];Chinese Journal of Animal Nutrition;2010-05
7 ZHANG Yuan-yuan1,QING Xian-ni1,LI Xi-hong1,WANG Qin-hong2(1.Faculty of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)(2.Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China);Response Surface Methodology for Optimization of cic,cis-muconic Acid Production by Escherichia coli[J];Modern Food Science and Technology;2011-04
8 LI Yu-zhen1,XIAO Huai-qiu1,YANG Tao2,LIN Qin-lu2(1.Applied Chemistry Department of Hunan Chemical Industry and Vocational Technology College,Zhuzhou 412004,Hunan;2.Food Science and Engineering College,Central South University of Forestry and Technology,Changsha 410004,Hunan,China);Response Surface Methodology for Optimization of Soybean Peptide Production Technology from Low-valued Soybean Meal[J];Soybean Science;2012-04
9 YANG Sheng;ZHANG Li-ping;FAN Huan;QIAO Jia-yun;WANG Wen-jie;College of Animal Science and Technology,Gansu Agricultural University;Tianjin Animal Husbandry and Veterinary Medicine Institute;;Optimization of culture medium and conditions of lactic acid bacteria in fermentation forage for fattening pig[J];Journal of Gansu Agricultural University;2013-04
10 MA Zhen-gui1,MA Wei-chao2,AN Jian-ping2,YANG Ling-juan2,LIANG Hong-yan2 (1.School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Gansu 730000,PRC; 2.College of Bioscience and Chemistry,Tianshui Normal University,Gansu 741000,PRC);Application of Response Surface Methodology in Production of Bacterial Protein from Potato Residue[J];Hunan Agricultural Sciences;2011-19
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XIONG Ming-yong,CHEN Ning,ZHANG Ke-xu (College of Food Science and Bioengineering, Tianjin University of Science and Technology, Tianjin 300222);Breeding of L-Valine-producing Strain and Medium Optimization of Fermentation Based on Pattern Recognition Method[J];Amino Acids & Biotic Resources;2003-01
2 FENG Wei quanTeachingandResearchSectionofExerciseBiochemistry,BUPE,Beijing100084,China;Characteristics of Metabolism of some Amino Acids and Sports Nutrition ——Development of Exercise Biochemistry(2)[J];JOURNAL OF BEIJING UNIVERSITY OF PHYSICAL EDVCATION;2000-03
3 Liu Fan1,Feng Xionghan1,Chen Xiuhua1,Qiu Guohong1,Tan Wenfeng1,He Jizheng21.Key Laboratory of Subtropical Agricultural Resource and Environment,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China2.Research Centre for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Advances in the study of biological genesis of manganese oxide minerals and their characteristics.[J];Earth Science Frontiers;2008-06
4 YANG Hong1,LI Dong1,ZHANG Jie1,CHEN Lixue2,ZHAO Yingli3(1. School of Architectural Engineering, Beijing Polytechnic University, Beijing 100022, China; 2. Northeast China Municipal Engineering Design and Research Institute, Changchun 130021, China;3.Shenyang Water Supply Company, Shenyang 110015, China );Mechanism of Biological Manganese Fixation and Removal and Biological Iron and Manganese Removal Technology from Ground Water[J];China Water & Wastewater;2003-06
5 Hong Jiaming(First People's Hospital of Zhaoqing. Guangdong Province, Zhaoqing 526021).Li Zengxi(Guangdong Institute of Analysis );Study on Relationship between Parkinsons' Disease and Trace Elements in Human Hair[J];TRACE ELEMENTS SCIENCE;1997-06
6 Li Shuang,Zhang Jian,Xie Yuanfeng,Wu Wen,Li Wenlian (Shanghai Institute of Industrial Microbiology,Shanghai 200233);Fermentation Conditions for Prducing Glutamine[J];INDUSTRIAL MICROBIOLOGY;1998-04
7 ZHONG Wei-zhang,LUO Yi-jing,ZHANG Zhong-zhi,LU Mang(Faculty of Chemical Science and Engineering,China University of Petroleum,Beijing 102249,China);Optimization of Liquid Medium for Rhodospirillum Branch of Photosynthetic Bacterium[J];Chemistry & Bioengineering;2009-07
8 LU Yan1, MEI Lehe1, LU Yuefei1, SHENG Qing2, YAO Shanjing1 (1Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China; 2School of Life Science , Zhejiang University of Science and Technology, Hangzhou 310037,Zhejiang, China);Optimization of P450 BM-3 activity produced by gene engineering strain by response surface methodology[J];Journal of Chemical Industry and Engineering(China);2006-05
9 JIANG Yuan-xiang, GAO Shu-hong, CHEN Chang-hua(State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China);Optimization of Medium Components for Adenosine Production by Bacillus subtilis Using Response Surface Design[J];Journal of East China University of Science and Technology;2005-03
10 Jiang Liyan Li Xue Lu Jiahui Meng Qingfan Teng Lirong (College of Life Science,Jilin University,Changchun 130012,China);Statistical Optimization of Fermentation Medium for Nisin[J];Chemical Reaction Engineering and Technology;2008-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved