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《Acta Biophysica Sinica》 1993-03
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A STUDY ON DNA DAMAGE INDUCED BY LIPID PEROXIDATION OF LINOLEIC ACID SYSTEM

Liu Xiaoqi Cao Enhua Zhu YuRong (Institute of Biophysics, Academia Sinica, Beijing 100101)  
Calf thymus DNA was reacted with peroxidizing linoleic acid at 37 C for 120 hours. DNA - peroxidizing linoleic acid fluorescent products increased with increasing of reaction time. Characteristic fluorescence spectra of these products with maximum excitation at 315nm and maximum emission at 420nm were obtained. Structural changes were occurred in DNA reacted with peroxidizing linoleic acid, as observed by decreased percentage of double-strand DNA. decreased fluorescence of DNA - EB complex with EB as the fluorescent probe.
【Fund】: 国家自然科学基金资助项目
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