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《Acta Biophysica Sinica》 2011-06
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Double Effect Intensity of 50 Hz Sinusoidal Electromagnetic Fields at Different Intensity on Differentiation and mRNA Expression of Osteoblasts in Vitro

ZHOU Jian1,CHEN Keming1,GE Baofeng1,CHENG Guozheng1,WANG Jiaqi1,WEI Zhe2 1.Institute of Orthopaedics,Lanzhou General Hospital of PLA,Lanzhou 730050,China; 2.Institute of Medical Engineering,Lanzhou General Hospital of PLA,Lanzhou 730050,China  
To investigate the effects of 50 Hz sinusoidal electromagnetic fields(SEMFs) at different intensity on differentiation and gene expression of osteoblasts in vitro.The newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 15 groups after one passage.The intensities of the SEMFs were 0 mT(control) and 0.9~4.8 mT(SEMFs groups,interval 0.3 mT) respectively,and the frequency was 50 Hz.The cells were exposed in the SEMFs for 30 min/d.Those without SEMFs exposure were used as control.The cells were observed under the contrast phase microscope each day.The calcified nodules were stained by vonkossa.After first treatment in SEMFs,bone morphogenetic protein-2(BMP-2),alkaline phosphatase(ALP) and osterix(OSX) mRNA expression were analysed at 0,12,24 and 96 h,respectively.The SEMFs groups were arranged in spiral appearance after 3~5 d.The calcified nodule numbers of 1.8 and 3.6 mT groups were significantly increased,ALP activity accord was the same as the result of calcified nodule numbers.The SEMFs treatment groups had different results,but there was a common result that 1.5,1.8,3.0 and 3.6 mT significantly increased the ALP,BMP-2 and OSX mRNA expression.The SEMFs at 50 Hz and 0.9~3.0 mT enhanced the maturation,mineralization and BMP-2,ALP and OSX mRNA expression of the osteoblasts,and SEMFs at 1.8 and 3.6 mT had the strongest activity.
【Fund】: 甘肃省科技重大专项资助项目(09ZNKDA025)~~
【CateGory Index】: R580;R683
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【Co-citations】
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