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《Journal of Biology》 2008-05
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Optimal conditions of extracting protein from shrimp shell by sodium hydroxide

HU Yun-hu,TU Jun-fang,HAO Pei-ying,WANG Lei(Department of chemistry and biology,Huainan Normal University,Huainan 232001,China)  
This research was designed to separate proteins from the shrimp shell according to the principle of salting-out of protein.The single factor analysis,such as the temperature,the concentration of NaOH and the reaction time was firstly carried out,followed by the orthogonal experiment analysis of three influencing factors and three levels to determine the best extracting condition of protein from the shrimp shell.The result showed that the optimal extracting conditions were:100℃,10% sodium hydroxide for 4 h.The protein purity was as high as 77.38%.
【Fund】: 淮南师范学院青年教师科学研究项目(2007lkq01)
【CateGory Index】: TS254.9
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