Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHINESE JOURNAL OF BIOLOGICALS》 1997-02
Add to Favorite Get Latest Update

Pilot Fermention Process of Secretory Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor

Lin Dianhai; Yang Guiyun; Chi Chunping et al (Changchun Institute of Biological Producls,ChangchuyJ 130062)  
The culture and expression coditions of secretory recombinant E. coli W3100/pGM-CSF were optimized during the pilot fermention process. More than 25 % (about 1. 5g/L) of total somatic proteins of secrtory GM-CSF were expressed in W3100 culture in 30L fermenter under the improved conditions. The specific activities of product reached over 2.0×107/ mg protein after being purified,
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 LIU Dongmei, ZHANG Zhuoyuan, WANG Yan ( Changchun Institute of Biological Products, Changchun 130062);Construction of Expression Vector of Secretory rhGM-CSF/IL-2 Fusion Protein[J];Chinese Journal of Biologicals;2002-04
2 CHI Chun ping,WANG Yan,LIU Shu ling,et al.(Changchun Institute of Biological Products,Changchun 130062);Quality Analysis of Secretory rhGM CSF during pilot fermention and purification process[J];Progress In Microbiology and Immunology;2001-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yi * , QU Xian Ming, LU Jian Feng, YANG Sheng Li ( Shanghai Research Center of Biotechnology, the Chinese Academy of Sciences, Shanghai 200233, China );Purification of Recombinant GM-CSF/IL-3 Fusion Protein[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;2000-03
2 Ling Mingsheng Zou Minji Xu Mingbo Wang Jiaxi Ma Xiankai (Institute of Basic Medical Sciences % Academy of Military Medical Sciences, Beijing 100850);Purification of Recombinant Human Granulocyte-macrophage Colony Stimulating Factor Expressed in Escherichia coli[J];Chinese Journal of Biotechnology;1995-03
3 He Cheng\ Zhu Yunsong (Dept. of Molecular Genetics, Shanghai Medical University\ Shanghai, 200032 China);Advances in the studies of methylotrophic yeast as a foreign gene expression system[J];PROGRESS IN BIOTECHNOLOGY;1998-03
4 LIU Li,XU Qi,HU Xiaoming,LIU Lizhong,WANG Ying,XIE Baoshu,LENG Aijun (Research Center of Molecular Biology,Airforce Hospital,Beijing 100036);Two 5′IL6 TNFα Mutant Fusion Proteins with SAPGTP and PG Linkers[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-04
5 HE Ping,TANG Zhaoyou,LIU Binbin,YE Shenglong (Liver Cancer Institute,Zhong Shan Hospital,Shanghai Medical University,Shanghai 200032);The Targeted Expression of Human IL 2/IFNα2b Fused Gene in Hepatoma Cells[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-04
6 JIANG Tai jiao,JI Xin song,ZHANG Ru an,YUAN Zhong yi (Shanghai Institute of Biochemistry,Chinese Academy of Sciences,Shanghai 200031,China);The Intracellular Production of Active Horseradish Peroxidase in Methyltrophic Yeast Pichia pastoris[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;2000-02
7 LI Ying hua 2) , BAI Jun jie 1)* , LI Xin hui 1) , YE Xing 1) , JIAN Qing 1) , LUO Jian ren 1) , LIANG Xu fang 2) ( 1) Pearl River Fisheries Research Institute, CAFS, Key Laboratory of Tropical & Subtropical Fis;Expression of Common Carp Growth Hormone in Yeast P. pastoris[J];Chinese Journal of Biochemistry and Molecular Biology;2001-04
8 DENG Bing\|bing, FANG Hong\|qing, XUE Chong, ZHAO Hong\|liang, LIU Zhi\|min\+* (Beijing Institute of Biotechnology,Beijing\ 100071,China);Secretory Expression of the Deleted Variant of Human Hepatocyte Growth Factor(hdHGF) in Pichia pastoris[J];Chinese Journal of Biochemistry and Molecular Biology;2001-05
9 HUANG Xiu-dong,YANG Hong 2,ZHANG Gao-xia 1,LI Shu-gang 1,YU Ting-he 3 (1 Chongqing Rain Biotech Institute,Chongqing 400060;2 Bioengineering Institute of Chongqing University,Chongqing 400044;3 Institute of Ultrasound Engineering in Medicine,Ch;Colorimetric assay of methanol in fermentation broth of Pichia pastoris[J];Biotechnology;2001-02
10 Li Fusheng; Zhang Zhiqing; Shao Hua et al (National Laboratory of Molecular Virology and Genetic Engineering, Beijing 100052);Study on Biological Activity of GM-CSF (12-127) Mutant[J];Chinese Journal of Biologicals;1996-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 YAN Lin QING Liuting BEI Weicheng HUANG Hong-liang CHEN Huan-chun(Laboratory of Animal Virology,College of Veterinary Medicine,Huazhong Agricultural University Wuhan 430070,China);Expression of Fusion Protein pIL6-IL2 in E.coli and Bio-activity Assay[J];Progress In Biotechnology;2004-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved