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Stability of Biological Characteristics of Recombinant Bacterial Strain Ex-Tumor Targeted IFNα2a

MENG Jieru,YAN Zhen,ZHAO Ning et al (Biotechnology Center, The Fourth Military Medical University, Xi'an 710032)  
Objective To study the stability of biological characteristics of recombinant bacterial strain pBV220-IFNN/DH5α.Methods The recombinant strain was subcultured for 50 passages, and the expression level of IFN,property of plasmid,as well as morphology, cultivation characters and biochemical reactions of various passages were studied. Results pBV220-IFNN/DH5α showed a typical morphology of E. coli, and the characteristics of various passages were not significantly different from that of primary strain. Conclusion The strain is suitable for large-scale production.
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