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Preliminary Study on Condition for Inactivation of Vibrio by Formalin

TAO Jia-fa,LAI Ying-tiao,REN Yan,KANG Guang-hui,ZHANG You,SHI Cun-bin,WU Shu-qin(PearlRiver Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangdong Key Laboratory ofAquatic Animal Immune Techniques,Guangzhou 510380,China)  
Objective To preliminarily study the condition for inactivation of vibrio,and provide parameters for development of production procedure for inactivated vibrio vaccine.Methods The culture liquid of vibrio was added with formalin at various concentrations and determined for time needed for activation at various temperatures.The vibrio was cultured in shake-flask and fermenter at 28℃ respectively,based on which the inactivation effects were compared,and the safeties of bulks of inactivated vaccine were evaluated.Results The minimum inhibitory concentration of formalin to vibrio was 0.025%.The times needed for inactivation with formalin at various concentrations and temperatures were different,which were shortened with the increasing formalin concentration and the increasing temperature.The inactivation effects in shake-flask and fermenter showed that vibrio was completely inactivated with 0.3% formalin at 28℃ for 24 h,and the safety of inactivated bulk met the relevant requirements.Conclusion The inactivation with 0.3% formalin at 28℃ for 24 h was suitable for vibrio.
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