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《Chinese Journal of Biologicals》 2011-11
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Effect of Pu-erh Tea Extract on Secretion of Tumor Necrosis Factor-α by Macrophages

LI Chun-lei,WANG Xuan-jun,SONG Shuang,LI Dong-qing,SHI Si,HAO Shu-mei,SHENG Jun(Changchun University of Science and Technology,Changchun 130022,China)  
Objective To investigate the effect of Pu-erh tea extract on secretion of tumor necrosis factor-α(TNF-α) by macrophages.Methods Macrophages were differentiated from THP-1 cells after treatment with phorbol ester and used as the model of inflammatory cells,which were treated by the mixture of lipopolysaccharide(LPS) at a final concentration of 100 EU / ml with Pu-erh tea extract at various concentrations(125 and 250 μg / ml) and determined for TNF-α content in cell culture by ELISA.Results Pu-erh tea extract effectively inhibited the secretion of TNF-α by macrophages.However,the inhibitory effect of 250 μg / ml Pu-erh tea extract was stronger than that of 125 μg / ml extract.Conclusion Pu-erh tea extract showed dose-dependent inhibitory effect on secretion of TNF-α by macrophages,and the effect was unassociated with EGCG,one of the major anti-inflammatory components of green tea.
【CateGory Index】: R285.5
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