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《Journal of Shanxi Agricultural Sciences》 2010-05
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Assessment of Genetic Diversity in Common Rice Oryza rufipogon

WANG Xing- chun1,YANG Zhi- rong1,WEI Xing- hua2 (1.College of life Science,Shanxi Agricutural University,Taigu 030801,China; 2.State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China)  
For broadening the choice of parents in cross breeding to change current limited genetic basis, 30 SSR(simple sequence repeats)primer pairs that disperse on 12 chromosomes in rice were used to study genetic diversity among 20 Oryza rufi- pogon Griff rice varieties. All the 30 SSR markers generated polymorphic bands revealing 100% polymorphism. Total 220 poly- morphic bands were detected among 20 varieties. The number of alleles (A)per locus ranged from 3 to 11 with an average of 7.3 alleles. Cluster analysis showed that all cultivars could be classified into 3 groups, two abroad groups and one native group. Almost all materials collected from the same region could be clustered together. Some materials from various regions could also be clus-tered together indicating that these materials have close genetic relationship with gene communion. This indicated SSR markers play an important role in ecotype classification and study of genetic diversity. The choice of parents in cross breeding could not be based only on its ecotype.
【Fund】: 国家973计划资助项目(2004CB117201);; 浙江省重点科研国际合作项目(2006C24012)
【CateGory Index】: S511.9
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