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《Journal of Shanxi Agricultural University (Natural Science Edition)》 1983-01
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A STUDY OF THE REGENERATION OF PLANTLETS FROM SOYBEAN EXPLANT CULTURES

Chen Yun-zhao wang Yu-guo  
The purpose of this paper is to introduce the ways of how the young ture leaves and the epicotyls of soybeans are cultured into plantlets and discuss the effect of plant growth hormones on shoot differentiation from explant. The young true leaves explant of soybean are cultured in B_5 basal medium containing NAA or KT+NAA,epicotyls explants are cultured in the B_5 medium containing KT+NAA, they are able to dedifferentiate into callus tissues, then directly develop into plantlets. If young true leaves are cultured in the B_5 medium containingKT+NAA, the rate of development of plantlets is higher than that only containing NAA. However, in the B_5 medium which contains KT+NAA, the rate of plantlets developed from epicotyl is higher than that from true leaves. It is more effective that NAA can accelerate the differentiation roots from young true leaves than it inducts callus tissues and the differentiation of plantlets, but KT is beneficial to the induction of callus tissues and the differentiation of plantlets. In the regeneration of plantlets from soybean explant, the result in the KT+NAA is better than only in the KT. In the compound ratio of KT+NAA, plantlets are differentiated earlier in lower concentration of NAA than in higher concentration, and the rate is higher too, however, more roots are differentiated in the later.
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