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《Journal of Shaanxi Normal University(Natural Science Edition)》 2012-04
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Preparation of lysozyme based molecularly imprinted polymers and its chromatographic evaluation

HAN Yan-ting,MA Jin-qin,YUE Xuan-feng,ZHANG Jing(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, College of Chemistry and Chemical Engineering, Shaanxi Normal University,Xi′an 710062,Shaanxi,China)  
Lysozyme-based molecularly imprinted polymers(Lys-MIPs) were prepared with PGMA/EDMA beads as a carrier material,lysozyme as the template molecule,β-cyclodextrin and acrylamide as the double functional monomers,N,N′-methylene bisacrylamide as the crosslinker.The prepared Lys-MIPs were used as a new HPLC packing material,based on which the related study on its recognition was conducted through the frontier chromatography.The results showed that Lys-MIPs had specific recognition for lysozyme,which helped achieve the separation between lysozyme and three individual competitive proteins,including bovine serum albumin(BSA),ovalbumin(OB) and myoglobin(Myo).
【Fund】: 国家自然科学基金资助项目(81173025)
【CateGory Index】: O631.3
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