Research on the Expression of IL-8 in PMVEC Directly-Induced by LPS and Its Mechanism through the Nuclear Factor-kappa B
FENG Guo-ji,GU Da-yong,CHEN Li, MA Bu-ren, YANG Ji-yun,ZENG Xiang-yuan (No. 401 Hospital of PLA,Shandong Qingdao 266071 .China; Research Institute of Surgery Raping Hospital,the Third Military Medical University, Chongqing 400042 China; Institute of Clinical Laboratory, Chengdu General Hospital of Chengdu Command of PLA,Sichuan Chengdu 610083,China)
Objective To study IL-8 expression of pulmonary microvascular endothelial cells (PMVEC)directly induced by. LPS and its mechanism through the nuclear factor-kappa B. Methods When PMVEC was directly excited by 100ng/ml LPS at 0,0. 5,1,2,4,6,8 h or 1,10,100 ng/ml LPS at 1h or 6h,the expressions of IL-8mRNA in PMVEC and its protein secretion in the supernatant of culture medium were examined by the methods of ISH, ELISA respectively and the activation of NF-kB was detected by EMSA. And those were then to be compared with which was inhibited by PDTC. Results LPS could directly act on the PMVEC to promote the IL-8 expression,including promoting the expression of IL-8mRNA and secretion of IL-8 protein,and the mRNA expressions increased before the protein secreted in terms of time. The LPS excitement could also significantly promote the activation of NF-kB,which peaked at 1h and then went down. PDTC could significantly decrease the expression of IL-8 and the activation of NF-kB. Conclusion The directly-induction of LPS could exactly promote the activation of NF-獴,then cause to the expression and secretion of IL-8. So as to provide the required physical condition for the PMN migration. It could be an important role in inflammatory response induced by LPS.
【CateGory Index】： R563