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《Journal of Shanxi Medical University》 2008-02
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Effect of bone marrow macrophage on T-bet expression in aplastic anemia mice

FAN Hai-bo,ZHANG Xiu-lian,ZHANG Wei-hua,FAN Xing-huo,CHEN Wei,HOU Su-min,LIU Chen(Dept of Hematology,First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China)  
Objective To study the expression of transcription factor T-bet of T helper 1 type cells in peripheral blood mononuclear cells(PBMNC) in aplastic anemia mice,and to explore the effect of mice bone marrow macrophage on T-bet in PBMNC of normal mice.Methods ①Immunologically mediated method was used to establish the model of aplastic anemia in BALB/C mice.②The expression of T-bet mRNA in PBMNC in aplastic mice was measured by reverse transcriptase polymerase chain reaction(RT-PCR).③PBMNC in normal mice was stimulated by supernatant of cultured myeloid matrix cells in aplastic mice and mouse bone marrow macrophage cell lines Ana-1 respectively,and the expression of T-bet mRNA was measured by RT-PCR.Results①The expression of T-bet mRNA in PBMNC in aplastic mice(1.49±0.18) was higher than in normal mice(1.03±0.06)(P0.05).②Stimulated by supernatant of cultured myeloid matrix cells in aplastic mice and mouse bone marrow macrophage cell lines Ana-1,the expression of T-bet mRNA in PBMNC of normal mice was 1.29±0.03 and 1.54±0.03,respectively,higher than that of normal controls(1.03±0.06)(P0.05).ConclusionThe results indicate macrophage may participate in immune pathogenesis of aplastic anemia through increasing the expression of T-bet.
【Fund】: 山西省高校科技研究开发基金资助项目(20041006)
【CateGory Index】: R556.5
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