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Integration of hepatitis B virus DNA in human embryo blastomere

ZHANG Chang-jun1,YANG Jing-ning2,WANG Hua1,LUO Qing-bin1,PENG Hai-yin1,XIONG Jing1 (1Research Center of Reproductive Medicine,The Affiliated People's Hospital,Yunyang Medical College,Shiyan 442000,China;2Dept of Immunology,Yunyang Medical College; )  
Objective To investigate the hepatitis B virus(HBV)DNA integration in human embryo via the in vitro fertilization(IVF)technique,and to explore the feasibility of biopsy in embryo blastomere for preventing HBV vertical transmission. Methods Forty-three couples undergoing IVF were divided into three groups:group A(18 couples:husband with HBsAg positive or HBeAg positive,while wife with HBsAg negative and anti-HBs positive),group B(20 couples with HBsAg and HBeAg double positive)and group C(control group,5 couples with HBV negative).Fluorescence in situ hybridization(FISH)analysis using partial-length HBV DNA as the hybridization probe was performed to explore the existence and integration of HBV DNA in the blastomere from HBV patients. Results FISH results showed that integrations of HBV DNA were detected in two human embryos in group A and nine in group B,and no integration was detected in group C.In group B,some blastomere had multi-copy integration.Compared with group A,the integration percentage of HBV DNA was improved significantly in group B(P0.05).Integration of HBV DNA appeared only in some cells,even if they came from the same HBV-infected embryo. Conclusion Integration of HBV DNA can be found in embryos from HBV patients.Blastomeres from couples with HBsAg and HBeAg double positive are easily integrated by HBV.
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