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《Journal of Shanxi Medical University》 2009-01
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Effect of nerve growth factor and basic fibroblast growth factor on proliferation of human periodontal ligament cells

WU Yun-xia,SHI Jin,SUN Xiao-jun,NIE Rui(Dept of Stomatology,First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China)  
Objective To evaluate the effects of nerve growth factor(NGF)and basic fibroblast growth factor(bFGF)on proliferation of human periodontal ligament cells(hPDLCs)cultured in vitro.Methods Human periodontal ligament cells were cultured by explant separation method.The fifth to seventh generation hPDLCs were cultured with the different concentrations of NGF,bFGF and NGF+bFGF.The proliferation of hPDLCs was measured by MTT colorimetric assay.Data was analyzed by ANOVA.Results Proliferation of hPDLCs increased in a dose-dependent manner after cultured with NGF or bFGF.The NGF and bFGF had synergetic effect on proliferation of hPDLCs.Conclusion NGF and bFGF can be used as bioactive mediators for periodontal regeneration.
【CateGory Index】: R96
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