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《Journal of Modern Oncology》 2005-02
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Cyclooxygenase-2 specific inhibitor celecoxib inhibites proliferarion and induces apoptosis on human osteosarcoma cell line-HOS-8603

YU Yi ,CAI Lin,LIAO Zhi-chao. Department of Orthopaedics Zhongnan Hospital,Me dical College,Wuhan University,Wuhan 430071,China.  
Obiective To evaluate the effects and mechanisms of ac tion of celecoxib in inhibiting proliferarion and inducing apoptosis on human os teosarcoma cells.Methods The proliferarion of human osteo sarcoma cell line-HOS-8603 was measured by MTT assay.The lever of prostaglandin E2 secreted by osteosarcoma cell was detected by High Performance Liquid Chromat ogram assay.Apoptosis was measured using FACS.Results The inhibitory rate of celecoxib on HOS-8603 cell was 46.75 ± 7.697%,if HOS-8603 cell has been treated with 80umol/L celecoxib for 48 hours. By FACS, it was fou nd that the rate of apoptosis cells were increased up to 46.0%(control group was 1.3%). The result of Western Blot assay revealed that apoptosis rates of differ ent concentrations of celecoxib accorded with the content of Cyclooxygenase-2. But these effects of celecoxib on HOS-8603 cells can't be counteracted by prosta glandin E 2:When we added 1.5mg/L prostaglandin E 2 to cells that were treated with 80umol/L celecoxib,the inhibitory rate and apoptosis rate were 46.30 ± 6. 812% , 45.8%(control group was 1.3%). Conclusion Celecoxi b played an inhibitory effect and induced apoptosis on HOS-8603 cells. But these effects of celecoxib can't be counteracted by prostaglandin E2.So their proba ble mechanism had nothing to do with prostaglandin E 2.
【Fund】: 湖北省科技攻关计划基金资助 (项目编号: 2004AA304B08)
【CateGory Index】: R738.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Yimin1,2,HAN Guoge1,2,FAN Biao2,3,LI Qi1,2,LI Hua2,WEI Lei21Renmin Hospital of Wuhan University/First Clinical College of Wuhan University,Wuhan 430060,China2Dept.of Pathophysiogy,School of Medicine,Wuhan University,Wuhan 430071,China3 Zhongnan Hospital of Wuhan University/Second Clinical College of Wuhan University,Wuhan 430071,China;Mechanism and Effect of Celebrex on the Migration Ability of ECV 304 Cells[J];Medical Journal of Wuhan University;2007-04
【Co-references】
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1 PENG Ting-sheng * ,QIU Ju-shi,WU Hui-xi,LIANG Hui-zh en,LUO Can-qiao Department of Pathology,Sun Yat-se n Medical College,Sun Yat-sen University,Guangzhou 510080,P.R.China;Expressions of CD44s,MMP-9,and Ki-67:Possible Association with Invasion,Metastasis,and Recurrence of Osteosarcoma[J];Chinese Journal of Cancer;2002-07
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3 Wang Yan 1, Qiu Jushi 1, Qiao Hui 1, Liang Huizhen 1, Luo Canqiao 1, Wang Rui 2 ( 1Department of Pathology, Sun Yat-sen College of Medical Science, Sun Yat-sen University, Guangzhou 510089; 2533rd Hospital of PLA, Kunming 650224);Expression of hypoxia-inducible factor 1α, VEGF and p53 and their association with angiogenesis and prognosis in osteosarcoma[J];Chinese Journal of Clinical and Experimental Pathology;2004-02
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1 KONG Shi-qi1,YU Li-jie2,HU Yu-hua1,YIN Feng-ren1,JI Jian-wen1,GU Hong-tao2,WU Jian-liang1 (1Department of Neurosurgery,Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;2Department of Oral Pathology,Stomatology Hospital of Hebei Medical University,Shijiazhuang 050017,China);Expression of COX-2 and its relationship to angiogenesis in human brain gliomas[J];Chinese Journal of Clinical and Experimental Pathology;2008-03
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