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《Journal of Modern Oncology》 2006-03
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X-chromosomal inactivation skewing in blood cells in Chinese healthy females screened for the females predisposed to cancer.

LI Gang~1, SU Qin~ 1,2 , ZHANG Wei~1, et al.~1Department of Pathology, ~2Department of Pathology, Cancer Hospital , Chinese Academy of Medical University and Peking Union Medical College, Beijing 100021, China; ~3Department of Oncology, TangDu Hospital, the Fourth Military Medical University, Xi'an 710038, China.  
Objective To investigate whether age is associated with X-chromosomal inactivation skewing in blood cells in Chinese healthy females . Methods DNA was extracted from mononuclear blood cells from 401 Chinese healthy females. Exon 1 of androgen receptor (AR) gene was amplified, with its products from different alleles resolved on denaturing polyacrylamide gels and visualized by silver staining. Corrected ratio (CR) of the AR allele for each HpaⅡdigested and auto- control sample pair was calculated. All statistical tests were two-sided. Results Comparisons were made among three age groups: ① neonates, ②young women group of females 16 to 50 years old and, ③old women group of females 50 to 96 years old. With CR≥3 adopted as the criterion, the incidence of skewing was 4.3% ( 2 of 46) in neonates(P=0.532 vs young women group),7.8% ( 13 of 166) in young women group(P=0vs old women group),and 25.7% ( 37 of 144) in old women group(P=0.001vs neonates). When a more stringent criterion of CR≥10 for skewing was used, the incidence of skewing was 2.2% ( 1 of 46) in neonates(P=1vs young women group),1.2% ( 2 of 166) in young women group(P=0vs old women group),and 11.1% ( 16 of 144) in old women group(P=0vs neonates). Conclusion Age is associated with X-chromosomal inactivation skewing in blood cells in Chinese healthy females. More attention should be paid to X-chromosomal inactivation skewing in blood cells in Chinese healthy females screened for the ones predisposed to cancer.
【Fund】: 国家自然科学基金资助项目(No.30572125和No.30171052);; 陕西省卫生厅科研基金项目(02D02)
【CateGory Index】: R394
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