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《Journal of Modern Oncology》 2008-07
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Experimental study of enhanced cytotoxicity of Navelbine to human pulmonary carcinoma cells A549 with hyperthermia

KANG Yan-xia1,ZHANG He-long1,FENG Ying-ming1,WANG Hong-mei1,ZHANG Rong-qing2,ZHANG Dong-wei2,FENG Yan-guo1, HU Ling1,YANG Ji-hua11Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;2Xijing Hospital Fourth Military Medillal Vniversity,Xi'an 710032,China.  
Objective:To investigate the effect and mechanism of hyperthermia on sensitivity of Navelbine. Methods: The working concentrations of NVB against human pulmonary adenocarcinoma cells(A549) were determined by MTT assay that has been developed for quantitative evaluation of the proliferation of cells. Then hyperthermia and chemotherapy were used singly or concurrently and the cell survival rates were obtained at 24h. The anticancer effect was evaluated by Veleriote method. The changes in cell cycle of cells and the assessment of apoptosis rates after treated for 42℃ hyperthermia and NVB were analysed by flow cytometry (FCM). Results: The concentration was defined as that of IC10-IC20 at 24h. It turned out to be 10ug/ml. Both single 42℃ hyperthermia and single chemotherapy suppressed the proliferation of A549 cells(P0.05); Combination of 42℃ hyperthermia and chemotherapy suppressed proliferation and killed more cells than single chemotherapy and single hyperthermia(P0.01).Flow cytometry showed that 42℃ hyperthermia resulted in progressive decrease in cells in S phase; Single chemical drug arrested cell cycle progression in G2/M phase; Compared with the group of single chemotherapy, the proportion of S phase cells of the group of combined treatment of 42℃ hyperthermia and chemotherapy decreased while the proportions of G2/M phase cells increased. The apoptosis rates of the four groups (control group, hyperthermia group, chemotherapy group and combination group)were 1.5%,7.9%,13.9% and 29.1% respectively. Conclusion: 42℃ hyperthermia can obviously enhance the cytotoxicity of NVB; The anti-tumor mechanism may be related to disturbance of cell cycle.
【CateGory Index】: R734.2
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【References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Hong,WANG Yong-gang,DING Yun-xia,LIN Xu,ZHANG Yong,JI Hua,KANG Bao-guo,LIU Yue Department of Oncology,Kunming General Hospital of Chengdu Military Command of PLA,Kunming 650032,China;Clinical study on malignant hydrothorax or hydroperitoneum treated with intracavitary perfusion chemotherapy combined with local endogenetic thermotherapy[J];Journal of Modern Oncology;2009-10
【Citations】
Chinese Journal Full-text Database 4 Hits
1 LU Hong reviewing , CHEN Long bang checking (Department of Oncology, Nanjing General Hospital of Nanjing Command/Clinical School of Medical College of Nanjing University, Nanjing 210002, Jiangsu, China);Hyperthermia combine with other therapies for carcer therapy[J];Journal of Medical Postgraduates;2004-05
2 Li Cai-yun~1,Zheng Ying~2,Yang Ren-rong~1,Shu Mei-zhi~1(1.Department of Surgery;2.Department of Pharmacy,PLA 117th Hospital,Hangzhou 310004,China);Effect of Combined Use of Hyperthermia and Chemotherapy against Human Gastric Cancer Cells in vitro[J];Clinical Journal of Medical Officer;2006-05
3 SUN Sheng-jie,JIAO Shun-chang,WEI xiu-fangDepartment of Medical Oncology,PLA General Hospital,Beijing 100853,China.;Experimental study of enhanced cytotoxicity of cisplatin to human pulmonary carcinoma PLA-801D cells by 42℃ hyperthermia[J];Journal of Modern Oncology;2006-05
4 JIA Xiao-qin, WANG Zheng-bing, CHEN Bin, LI Hou-da(Department of Medicine of Yangzhou Medical College, Yangzhou University, The Co mparative Medicine Center of Yangzhou University, Yangzhou, 225001);APOPTOSIS EFFECTS OF RECOMBINANT TRAIL ON 7402 HEPATIC CELL LINE[J];Journal of Jiangsu Clinical Medicine;2004-06
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Zhou Xijing, Fang Qingfang, Qiu Youhao, et al. Zhejiang Province Tumor Hospital, Hang Zhou 310022;Clinical study of cisplatin injected into thoracic cavity combined with hyperthermotherapy in the treatment of pleural effusion from malignant neoplams[J];Chinese Clinical Oncology;2000-02
2 SUN Sheng-jie,JIAO Shun-chang,WEI xiu-fangDepartment of Medical Oncology,PLA General Hospital,Beijing 100853,China.;Experimental study of enhanced cytotoxicity of cisplatin to human pulmonary carcinoma PLA-801D cells by 42℃ hyperthermia[J];Journal of Modern Oncology;2006-05
3 MaKing;Cai Guiru Department of Obstetrics Gynecology, Tongji Hospital, Tongji Medical University, Wuhan;INHIBITORY EFFECT OF A-AS ON PROLIFERATION OF HUMAN OVARIAN CANCER CELLS IN VITRO[J];Tumor;1992-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Shengjie, JIAO Shunchang(Department of Medical Oncology,PLA General Hospital,Beijing 100853,China );Effect of combined use of hyperthermia and DDP in different time sequence on tumoricidal activity against human pulmonal giant cell carcinoma cells in vitro[J];Journal of Health Care and Medicine in Chinese PLA;2005-02
2 LU Hong, LI Bing, XIONG Xiao-bin, HU Xiao-lin, GAO Shu-ping, ZHANG Rui-zhong(Department of Radiation Oncology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, Jiangsu, China);Effects of intracavitary hyperthermia combined with external beam radiation and intraluminal brachytherapy in the treatment of esophageal carcinoma[J];Bulletin of Medical Postgraduate;2002-04
3 LU Jian-wei (Department of Oncology, Cancer Institute & Hospital of Jiangsu , Nanjing 210009);MULTI-CENTER CLINICAL TRAIL OF 5-Fu CONTINUOUS INTRAVENOUS INFUSION COMBINED WITH OCTREOTIDE IN THE TREATMENT OF ADVANCED PRIMARY HEPATIC CANCER[J];Journal of Jiangsu Clinical Medicine;2003-04
4 ZHU Jin 1, LI Ning-chuan 2, CHENG Hong 1(1. Medical college of Yangzhou University, Yangzhou, Jiangsu, 225001 2. College of Physical Education of Yangzhou University, Yangzhou, Jiangsu, 225002);MOLECULAR MECHANISM OF APOPTOSIS OF LEUKAMIC CELLS INDUCED BY SOLUBLE TRAIL[J];Journal of Jiangsu Clinical Medicine;2004-03
5 JIA Xiao-qin, WANG Zheng-bing, CHEN Bin, LI Hou-da(Department of Medicine of Yangzhou Medical College, Yangzhou University, The Co mparative Medicine Center of Yangzhou University, Yangzhou, 225001);APOPTOSIS EFFECTS OF RECOMBINANT TRAIL ON 7402 HEPATIC CELL LINE[J];Journal of Jiangsu Clinical Medicine;2004-06
6 YANG Yang,LIU Bao-rui,QIAN Xiao-ping(Department of Medical Oncology,Gulou Hospital Affiliated to Medical College of Nanjing University,Nanjing,Jiangsu,210008);RESEARCH ON COMBINATION OF CISPLATIN WITH HYPERTHERMIA AGAINST HEPATOCELLULAR CARCINOMA CELL LINE SMMC-7721 IN VITRO[J];Journal of Clinical Medicine in Practice;2006-05
7 XU Hai-dong,ZHOU Zhao-ying,ZHANG Dong-xu(Department of Precision Instruments and Machines,Tsinghua University,Beijing100084);Non-invasive temperature measurement[J];Medical Equipment Journal;2002-02
8 HUANG-Wei, ZHANG Yao-zhen, ZHOU Jian-feng, LIU Wen-li (Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China);Influence of DDP and ADM on cell cycle and apoptosis in K562 cells[J];Chinese Journal of Pathophysiology;2005-03
9 YANG Yao-qin,YANG Hu-chuan,TAO Hui-hong,et al. Tumor Cytology Research Unit,Medical College of Tongji University,Shanghai 200070,China;The antitumor effects of Tween-80 combined with hyperthermia in vivo and in vitro[J];Chinese Journal of Clinkal Oncology and Rehabilitation;2001-05
10 HE Chao,HU Xiaotong,XU Xiangming, FANG Bingliang. Clin ical Res earch Institute of Sir Run Shaw Hospital , Zhejiang University, Hangzhou 310016, China;Action of apoptosis-induced ligand gene in relation to tumor necrosis factor on human colon cancer cell line HT29[J];Chinese Journal of Oncology;2002-02
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