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《Journal of Modern Oncology》 2008-08
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Inhibitory effect of paridis on angiogenesis in vitro

HU Jing,QIAN Xiao-ping,LIU Bao-rui,ZHU Li-jing,HU Wen-jing,SUN Jing,WANG Li-feng,YU Li-xiaCancer Center of Nanjing University Medical College Affiliated Drum Tower Hospital,Nanjing 210008,China.  
Objective:To approach the inhibitory action and the mechanism of the alcohol extract of paridis to angiogenesis in vitro.Methods:The MTT assay was used to observed the effect of the alcohol extract of paridis to EC of human umbilical vein(HUVEC)and human intestine carcinoma LOVO cell.Transwell cabin test and out-body canaliculization test were used to observe the alcohol extract of paridis impact the migrational and vaso-formed ability of HUVEC.Results:When the concentration of the alcohol extract of paridis between 15μg/ml to 60μg/ml,the inhibition rate of EC was between 24.2% and 82.1%,and there was inhibitory action to vascular endothelial cell proliferation.When the concentration of the alcohol extract of paridis between 15μg/ml to 60μg/ml,numbers of canaliculization was reduced and lumens was unintegrity in out-body canaliculization test.And there was significant difference compared with the control group(P=0.0000011,0.0000417,0.0008752).And the numbers of endothelial cell migration was significant reduced than the control group(P=0.0000005,0.0000033,0.009993).There was no evident cell toxic action to human intestine carcinoma LOVE cell(the cell inhibition was between 12.1% to 13.9%)between the range of concentration.The alcohol extract of paridis can induce endothelial cell apoptosis,suppress endothelial cell DNA synthesis,and there was dose dependent(coefficient correlation 0.929 and-0.922,and P=0.036,0.039).Conclusion:The alcohol extract of paridis can active suppress the angiogenesis in vitro.The mechanism maybe concerned with inhibited proliferation,migration and canaliculization of endothelial cell,induced the apoptosis of endothelial cell and inhibited the synthesis of endothelial cell DNA.
【Fund】: 江苏省自然科学基金项目(BK2006005)
【CateGory Index】: R285.5
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