Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Modern Oncology》 2008-09
Add to Favorite Get Latest Update

Study on protective effects against tumor relapse induced by nanoliposome - encapsulated tumor specific antigen MAGE3/HSP70[NL(MH)]

LI Xia1,HU Pei-zhen1,ZHANG Xiu-min1,HUANG Yang2,SI Shao-yan3,SUI Yan-fang1,CHEN Juan41Department of Pathology,the Fourth Military Medical University,Xi'an 710032,China;2Department of Emergency,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,China;3306th Hospital of PLA,Beijing 100101,China;4Depatment of Urology Surgery,the Second Affiliated Hospital,Xi'an Jiaotong University,Xi'an 710004,China  
Objective:To evaluate the protective effects against tumor relapse induced by nanoliposome vaccine.Methods:The C57BL/6 mice were immunized with PBS,NL(nanoliposome - unencapsulated tumor specific antigen MAGE3/HSP70),MH/NL(MAGE3/HSP70 and NL),MH,NL(MH).The spleen lymphocytes secreting IFN-γ,the cyto-toxicity of CTL to B16-MAGE3 were measured by ELISPOT,LDH release assay to evaluate the cellular-activating effects.The protective and therapeutic effects against tumor metastasis were detected using artifical metastasis experiment mold and spontaneitic metastasis experiment mold;The protective effects against tumor relapse were detected using tumor cells challenge experiments and tumor cut experiment.Results:After the C57BL/6 mice were immunized with NL(MH),there was the most frequency of MAGE3 specific CTL,highest cytotoxicity of CTL to B16-MAGE3 cells.The metastasis tumor numbers of C57BL/6 mice were immunized with NL(MH) were minimum.Conclusion:NL(MH) can elicit MAGE3-specific cellular-immune response and antitumor effects against the MAGE3-expressing tumor.NL(MH) could significantly protect mice effectively from tumor relapse and metastasis.
【Fund】: 国家自然科学基金资助项目(No.30600552);; 国家高技术研究发展计划(863计划)资助项目(No.2007AA02Z470);; 陕西省自然科学基金资助项目(No.2007C202)
【CateGory Index】: R730.51
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved