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《Journal of Modern Oncology》 2010-01
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Expression and significance of epidermal growth factor receptor variant Ⅲ and matrix metalloproteinase-2 in human esophageal carcinoma

LIU Min1,GUO Xiao-juan1,ZHANG Hong-xia1,WANG Peng2,DUAN Xiao-yi3,GUO You-min3 1Department of Radiology,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China;2Imaging Center,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710004,China;3Imaging Center,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China.  
Objective:To detect the expression and relation of epidermal growth factor receptor variant Ⅲ(EGFRvⅢ)and matrix metalloproteinase-2(MMP-2)in the genesis and development of human esophageal carcinoma.Methods:In 66 human esophageal carcinoma tissues,the expression of EGFRvⅢ was detected by immunohistochemistry and Western-blot.MMP-2 was detected by immunohistochemistry.The relation between various clinicopathologic factors and EGFRvⅢ,MMP-2 was analyzed.Correlation analysis between EGFRvⅢ and MMP-2 was analyzed by Pearson correlation coefficient.Results:The average gray scale values of EGFRvⅢ in esophageal carcinoma and normal tissues by immunohistochemistry were 22.46±4.21 and 5.54±3.01,which showed a significant difference(t=6.701,P0.001).The average gray scale values by Western-blot were 0.83±0.15 and 0.083±0.049,which had a significant difference(t=9.362,P0.001).The average gray scale values of MMP-2 in esophageal carcinoma and normal tissues by immunohistochemistry were 37.46±6.18 and 9.80±2.24,which showed a significant difference(t=8.213,P0.001).Correlation analysis between EGFRvⅢ and MMP-2 showed r=0.917,P0.001,suggesting a good positive correlation.The significant differences were observed in TNM-stage,lymph node metastasis and classification in both EGFRvⅢ(P0.05)and MMP-2(P0.05),and no obvious difference in sex,age,tumor size,growing type(P0.05).Conclusion:EGFRvⅢ express in human esop hageal carcinoma.Combination of EGFRvIII and MMP-2 could be useful markers for tumorgenesis and differentiation of human esophageal carcinoma.
【Fund】: 国家自然科学基金资助项目(No.30770619 30600744)
【CateGory Index】: R735.1
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