Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Modern Oncology》 2013-02
Add to Favorite Get Latest Update

Morphological and functional changes of mitochondrial in apoptotic esophageal carcinoma cell induced by oridonin

Xin Qingfeng1,2,Chen Junhui2,Xie Xiaoyuan3,Shen Zhongying3 1The Third Affiliated Hosptal of Luohe Medical College,Henan Luohe 462000,China;2Peking University Shenzhen Hospital,Guangdong Shenzhen 518036,China;3Medical College,Shantou University,Guangdong Shantou 515031,China.  
Objective:To demonstrate that mitochondrial morphological and functional changes in the course of apoptosis in esophageal carcinoma cell induced by oridonin(ORI).Methods: The cultural esophageal carcinoma cell line SHEEC was treated with ORI(32μg/ml).After 2,4,6,12,24 hours of drug adding,the SHEEC cells were collected for TUNEL and electron microscopic examination.The mitochondria transmembrane potential(MTP,△Ψm) change was calculated by Rhodamine 123(Rho123) fluorescence probe and the fluorescent intensity of the mitochondria was measured by flowcytometer and cytofluorimetric analysis.Results: In the early-stage(2h) after adding oridonin an adaptive proliferation of mitochondria appeared;In the mid-stage(4h),the mitochondria swelled with outer membrane broken;Then after 8 hours,the cell nucleus showed typical apoptotic changes.After 24 hours,the fluorescent intensity decreased.Conclusion: Under the inducement of oridonin,the apoptotic changes of SHEEC cells were apparent morphological and functional changes of mitochondria with decrease of mitochondrial transmembrane potential.It is considered that changes of mitochondrial are important intermediate link in the course of aopotosis of esophageal carcinoma cells induced by ORI.
【Fund】: 国家中医药管理局中医药科学技术研究专项课题(编号:06-07ZP35);; 广东省科技计划资助项目(编号:2006B35630009)
【CateGory Index】: R735.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Xin Qingfeng1,Chen Junhui2,Xie Xiaoyuan3(1 Luohe Medical college,Luohe 462000;2 Peking University Shenzhen Hospital,Shenzhen 518036;3 Medical College,Shantou University,Shantou 515031);Changes in mitochondrial and intracellular calcium ions in process of SHEEC cells apoptosis induced by oridonin[J];Pharmacology and Clinics of Chinese Materia Medica;2013-03
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Junhui Chen1 Shaobin Wang1 Dongyang Chen1 Guisheng Chang1 Qingfeng Xin1 Shoujun Yuan2 Zhongying Shen3 1 Department of Oncology, The First Affi liated Hospital of Shantou University Medical Col- lege, Shantou 515041, China. 2 Pharmacology Laboratory, Institute of Ra- diation Medicine, Military Medical Sciences Academy of Chinese PLA, Beijing 100270, China 3 Department of Pathology, Shantou Univer- sity Medical College, Shantou 515031, China.;The Inhibitory Effect of Oridonin on the Growth of Fifteen Human Cancer Cell Lines[J];中国肿瘤临床(英文版);2007-01
2 XIE Xiao-yuan1,CHEN Jun-hui1,WANG Shao-bin2,LIU Yu-xun2,SHEN Zhong-ying2 1Peking University Shenzhen Hospital,Shenzhen 518036,China;2Shantou University Medical College,Shantou 515031,China.;The apoptosis of human esophageal carcinoma cell line SHEE and the change of mitochondria induced by oridonin[J];Journal of Modern Oncology;2008-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Sheng-li, HAN Shao-yin, ZHANG Qiao, DING Lan-ping, SONG Ai-yun, GONG Ya-ou, ZHANG Tan-mu (Department of Esophageal Cancer Research, Henan Medical University, Zhengzhou 450052, China);STUDY ON THE ANTIMUTAGENICTTY OF ORIDONIN[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-01
2 CHENG Ying et al(Natural Products Utilization Research Unit,Leshan Normal College,Leshan,Sichuan 614004);Effects of Polysaccharides from Potentilla anserina on the Levels of Cytokines in Tumor Mice[J];Journal of Anhui Agricultural Sciences;2012-09
3 HUANG Tao1, ZHENG Yu-ling2, SUN Tai-zhen2, XU Hai-ying1 (1Medical School of Huanghe Science and Technology College, Zhengzhou 450005,China; 2Department of Internal Medicine, Henan University of TCM, Zhengzhou 450008, China);Effect of Dihuang Guanshitong Oral Liquids on expression of E-cadherin of esophageal carcinoma rats[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-11
4 WANG Zhi-hong1,XUE Jian-bin2,JIANG Wen-yan2,ZHANG Gui-rong2(1.Basic Medical College,Changchun University of Chinese Medicine,Changchun 130117,China; 2.Life Science College,Jilin University,Changchun 130012,China);A study on effects of Menispermum dauricum alkali polysaccharide on Hela cell proliferation[J];Journal of Northeast Normal University(Natural Science Edition);2011-04
5 Shen Wen1,Xu Jian 1*,Sun Jinquan1,et al 1.Life Science College,Zhejiang Chinese Medical University,Hangzhou(310053),China;2.Sir Run Run Shaw Hospital;Study on Cyclin D/Rb/p16 Signal Pathway of BxPC-3 Cell Line Treated with Oridonin[J];Journal of Zhejiang Chinese Medical University;2013-05
6 LIU Jing,LIANG Jing yu *,XIE Tao(Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009;china);Development of Rabdosia Rubescens〔Hemsl.〕Hara[J];Strait Pharmaceutical Journal;2004-02
7 Li Ruifang,Wang Qingduan (Henan Institut of Medical Science,Zhengzhou 450052,China);Regulation of Telomerase Activity and Cell Cycle by Oridonin in K562 Cells[J];Henan Journal of Oncology;2004-05
8 Yan Jianting 1, Yang Shengli 2, Gong Ya'ou 2,Ding Lanping 2,Song Aiyun 2(1.Architectural School of Henan Province, Zhengzhou 450007, China;2.Department of Esophageal Cancer Research,Basic Medical College,Zhengzhou University,Zhengzhou 450052,China);The Effect of Oridonin on DNA Damage in the Mammalian Cells[J];Henan Journal of Oncology;2005-02
9 Wang Mianying Department of Pharmacology, Henan Institute of Medical Sciences;The experimental study of the chemotherapy scheme on ECA, S180 and P388 leukaemia with the combination of oridonin, vincristine and cisdiamminedichloroplatinum[J];;1987-03
10 Wang qingduan et al Henan Institute of Medical Sciences;Study of the antitumor action of lushanrubescensine A[J];;1987-04
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 XIE Xiao-yuan1,CHEN Jun-hui1,WANG Shao-bin2,LIU Yu-xun2,SHEN Zhong-ying2 1Peking University Shenzhen Hospital,Shenzhen 518036,China;2Shantou University Medical College,Shantou 515031,China.;The apoptosis of human esophageal carcinoma cell line SHEE and the change of mitochondria induced by oridonin[J];Journal of Modern Oncology;2008-06
2 XIN Qing-feng,CHEN Jun-hui(Peking University Shenzhen Hospital, Shenzhen 518036,China)WANG Shao-bin,LIU Yu-xun,SHEN Zhong-ying(Medical College, Shantou University, Shantou 515031,China);Proliferation Inhibition and Apoptosis of Human Gastric Cancer Cells Induced by Oridnin[J];China Pharmacy;2008-15
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 Li Ruifang,Wang Qingduan (Henan Institut of Medical Science,Zhengzhou 450052,China);Regulation of Telomerase Activity and Cell Cycle by Oridonin in K562 Cells[J];Henan Journal of Oncology;2004-05
2 Li Qin, Feng Weisheng Henan College of Traditional Chinese Medicine;The Latest Development of Researches on the Chemical Constituents and Pharmacological Activity of Rabdosia rubescens (Hemls.) Hara[J];Journal of Henan College of Traditional Chinese Medicine;2003-06
3 LIN Jue long(Central Laboratory,Medical College of Shantou University,Shantou 515031,China);Study on Action of Flow Cytometry on Apoptotic Cell[J];Modern Diagnosis & Treatment;2002-04
4 ZHANG Chun-ling 1,2, WU Li-jun2, ZUO Hai-jun2, TASHIRO Shin-ichi3, ONODERA Satoshi3, IKEJIMA Takashi 1 (1.China-Japan Research Institute of Medical and Pharmaceutical Sciences; 2. Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo 194-8543, Japan);Oridonin-induced A375-S2 cell apoptosis through mitochondrial pathway[J];Chinese Traditional and Herbal Drugs;2004-04
5 Chun-ling ZHANG1,2, Li-jun WU2, Shin-ichi TASHIRO3, Satoshi ONODERA3, Takashi IKEJIMA1,41China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University,Shenyang 110016, China; 2Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang 110016, China;3Department of Clinical and Biomedical Sciences, Showa College of Pharmaceutical Sciences, Tokyo 194-8543, Japan prof Takashi IKEJIMA.;Oridonin induces apoptosis of HeLa cells via altering expression of Bcl-2/Bax and activating caspase-3/ICAD pathway[J];中国药理学报(英文版);2004-05
6 LIU Yan-qiu~(1,2),YOU Song~(2),TASHIRO Shin-ichi~(3),ONODERA Satoshi~(3),IKEJIMA Takashi~(1)(1.China-Japan Research Institute of Medical and Pharmaceutical Sciences,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Laboratory of Natural Product Biotechnology,Shenyang Pharmaceutical University,Shenyang 110016,China;3.Department of Clinical and Biomedical Sciences,Showa Pharmaceutical University,Machida,Tokyo 194-8543,Japan);Oridonin induced U937 cell apoptosis through ERK pathway[J];China Journal of Chinese Materia Medica;2005-23
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved