Study of β-elemene on induction of apoptosis and reverses on tamoxifen drug-resistance in human breast cancer cells LCC-2

Ma Hailin;Zhou Xiaojuan;Zhang Xiaozhi;Wang Tao;Department of Radiation Oncology,First Affiliated Hospital,Medical College of Xi'an Jiaotong University;Department of Oncology,First Affiliated Hospital,Medical College of Xi'an Jiaotong University;  

Objective:To study the effect of β- elemene on induction of apoptosis and reverses on tamoxifen(TAM) resistance in human breast cancer cell line LCC-2. Methods:The sensitivity of drug and the reversal of drug- resistance were examined by using MTT assay,the cell cycle and apoptosis were detected by flows cytometry,expression of Bcl- 2 protein and pS2 protein were detected by SP method. Results:β- elemene could decrease the IC50 value of LCC- 2 cell on tamoxifen,with a reversal of 3. 6 fold,influence cell cycle,obviously strengthens. The effect of inducing apoptosis of LCC- 2 cell. The apoptosis percentage was increased from(2. 17 ± 0. 16)% to(9. 13± 0. 34)%. The expression of Bcl- 2 protein was reduced from(56. 52 ± 4. 85)% to(28. 02 ± 6. 32)%,expression of pS2 was negative. Conclusion:β- elemene could partially reverse tamoxifen resistance of LCC- 2 cell line,its reversal mechanism mainly related to suppress of the expression of Bcl- 2,induces apoptosis of drug- resistance cell to reverses MDR.

【Key Words】： β-elemene breast cancer LCC2 apoptosis reverse erug-resistance
【CateGory Index】： R737.9

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