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《Journal of Modern Oncology》 2015-01
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MicroRNA- 100 regulates Hep G2 cell migration,invasion and EMT process by regulating mTOR gene expression

Sui Chengguang;Meng Fandong;Fu Liye;The Second Cancer Research Institute,The First Hospital of China Medical University;  
Objective: To investigate the effect of microRNA- 100 on the migration,invasion and epithelial- mesenchymal transition( EMT) process of human hepatocarcinoma Hep G2 cells and the possible mechanism. Methods:Synthetic miRNA- 100 mimic and its negative control were transfected into Hep G2 cells by liposome method. We divided cells into three groups: mock group,control group and miRNA- 100 mimic group. After transfection,the expression of m TOR at mRNA level was detected by quantitative real- time PCR. The migration of Hep G2 cells was detected by wound scratch assay,and the invasion ability changes were analyzed in transwell. Expressions of MMP- 2,MMP- 9,N- cadherin,Vimentin,α- SMA and E- cadherin were analyzed by Western blot. Results: MiRNA- 100 can inhibit the migration and invasion ability via down- regulate the m TOR expression. The Western blot results showed that miRNA- 100 could down- regulate the expression of MMP- 2,MMP- 9,N- cadherin,Vimentin and α- SMA and up- regulated the expression of E- cadherin,and inhibit the progress of EMT. Conclusion: MiRNA-100 can suppress the migration and invasion process and EMT process,it is may be achieved by down- regulating m TOR gene expression.
【Fund】: 辽宁省科技发展计划重点项目(No.2008225008-5)
【CateGory Index】: R735.7
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