Proliferation Inhibition and Anti-invasion Effect of Retinoic Acid and Glycyrrhizin in Human Lung Cancer Cell Line

ZHANG Dong fang,HUANG Wei,HUANG Ji qun,et al. Cancer Institute,Guangzhou Medical College,Guangzhou,510182  

Objective To study the effects on proliferation inhibition and anti invasion of retinoic acid(RA) and glycyrrhizin(GL) single or combined,in highly metastasis lung cancer cell line(PGCL3).Methods The proliferation inhibitive rate,the colony formation rate in soft agar,the invasive ability to reconstituted basement membrane,the chemotatic migration ability,the laminin adhesion ability,and the activity of cathepsin B(CB) were tested.Results Treated with RA and GL,the proliferation of PGCL3 cells was inhibited obviously,and the inhibition degree was related to dose of the drugs.IC50 of the proliferation inhibition was 12.6 μmol/L and 1.8 mmol/L respectively.Treated with 2.5 μmol/L,5.0 μmol/L RA and 0.5 mmol/L,1.0 mmol/L GL,the invasive ability was decreased significantly( P 0.05 and P 0.01),and the inhibition was in a dose dependent manner.In combined treatment with 5.0 μmol/L RA and 0.5 mmol/L GL,the inhibition of invasion was greater than the sum of single treatment with the two drugs.Treated with GL of above concentration,the adhesion and migration ability,the secretion of CB and the colony formation rate in semi solid agar of the PGCL3 cells were decreased significantly( P 0.01).Conclusion RA and GL can inhibit the proliferation and invasion of the PGCL3 human lung cancer cells,and their effects of anti invasion is of synergism.The mechanism of anti invasion of GL is to inhibit many points of invasive process.

【Key Words】： Lung cancer cells Retinoic acid(RA) Glycyrrhizin Proliferation Invasion
【Fund】： 广州市科技攻关课题 (编号 :98-Z - 0 4 1 - 0 1 )
【CateGory Index】： R734.2

Download(CAJ format) Download(PDF format)

CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.