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《Laboratory Animal Science》 2007-03
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The Replication Characteristics of NDV-Lasota Strain in the CEF Cell

JIN Ji-chang,ZHAO Li-hong~*,QIAO Jian,HU Yong-feng,TIAN Yong,ZHAO Hui-ying,HOU Hai-yan(College of Veterinary Medicine,China Agricultural University,Beijing 100094)  
The replication characteristics of NDV-Lasota strain in CEF cell were evaluated by cytopathic effects(CPE),hemadsorption test,hemagglutination test,and plaque assay.The results showed that when LH maintenance media supplemented with 25 μg/mL trypsin,the virus replication was effective in CEF and developed dynamicly.The infected cell obtained the hemadsorption ability 24 hour post inoculation(h.p.i),the supernatant of culture medium was positive with HA test 30 h.p.i,obvious CPE appeared 48 h.p.i,and small clear plaque appeared on the monolayer of cell 72 h.p.i.
【Key Words】: NDV CEF Proliferation characteristics
【CateGory Index】: S855
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