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《Laboratory Animal Science》 2008-02
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Developing of The BVgD-Peptide ELISA for Detection of Antibodies Against Monkey B Virus

XIAO Jing~1,FU Rui~1,HE Zheng-ming~1,SHI Chang-jun~2(1.National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China)(2.VRL Laboratories-China Division,Xishan Biotechnology,Inc.,Suzhou 215111,China)  
Objective To develop ELISA for detection of antibodies against monkey B virus using BVgD-Peptide as antigen.Methods Antigenicity of BVgD-Peptide antigenic determinant was analyzed by the Western-blot method.The ELISA method was established by optimization of reaction condition and reagent selection.The parallel detection of monkey sera was performed by using three different ELISA kits using complete BV,HSV-1 and BVgD-Peptide as antigens.The detection results obtained by ELISA kit developed in the study were compared with the results obtained by ELISA kit made in USA.Results Compared with ELISA using as complete BV and HSV-1 antigens,ELISA using BVgD-Peptide as antigen was less sensitive but more specific.85% of the detection results obtained by ELISA kit developed in the study were coincident with those obtained form Laboratory of America.Conclusion ELISA for detection of antibodies against monkey B virus using BVgD-Peptide as antigen has been developed,and this ELISA is more specific than ELSA using complete BV and HSV-1 as antigens.
【Fund】: 北京市科委实验动物法制管理与科技支撑平台(课题编号:Z0004100040691-1)
【CateGory Index】: R446.6
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