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《Laboratory Animal Science》 2011-05
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The Study of Randomly Amplified Polymorphic DNA Characteristic in HFJ and MIJ Inbred Rats

CAI Yue-hua,LI Jian-hui,ZHENG Long,LIU Jun-xu,WANG Jun-xia,XU Zeng-nian,SHI Rong-zhen,LIU Fu-ying(Hebei Medical University,Hebei Key Lab of Laboratory Animal Science,Shijiazhuang 050017,China)  
Objective To establish the fingerprint of HFJ and MIJ inbred rats by randomly amplified polymorphic DNA(RAPD) analysis.And to compare the DNA polymorphism of the four inbreeds rats including HFJ,MIJ,Lewis and F344.Methods To isolate rats genomic DNA by standard procedure of phenol/chloroform.Sixty primers were used to amplify rats DNA with RAPD-PCR.Thirty primers could produce clear fragments and were selected to analyze the DNA polymorphism between HFJ and MIJ inbred rats.Twelve primers were selected to analyze the DNA polymorphism among HFJ,MIJ,Lewis and F344 inbred rats.Results 1~7 clear bands were detected by electrophoresis on agarose gel in HFJ and MIJ inbred rats with thirty primers respectively.All amplified bands by random oligonucleotide primers were located between 200 bp~1 100 bp except primer O~08,which located over 2 000 bp and there was a distinct band near the origin of electrophoresis.The results of RAPD indicated that there were identical completely between HFJ and MIJ inbred rats or within the two strains.DNA polymorphisms among HFJ,MIJ,Lewis and F344 inbred rats were studied by RAPD using twelve primers.The results indicated that 5 primers could to obtain difference fragments in all twelve primers.Conclusion With the thirty random primers to establish RAPD fingerprint of HFJ and MIJ inbred rats.Since the two inbred strains originated from a pair of Wistar rats,DNA polymorphism between HFJ and MIJ inbred rats were no detected,although the two inbred strains had remarkable genetic difference.By RAPD analysis,DNA polymorphism among HFJ,MIJ,Lewis and F344 inbred rats were detected.
【Fund】: 河北省科技支撑计划重点项目(No.09276426D)
【CateGory Index】: Q95-33
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