Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《LABORATORY ANIMAL SCIENCE AND ADMINISTRATION》 1996-03
Add to Favorite Get Latest Update

p53 Gene Mutation in a Benzo(a)Pyrene-induced Human Bronchial Oarcinoma Cell Line

Yang Zhaohai; Zheng Jie; Tao Jiangchuan; You Jiangfeng;Cui Xianglin;Fang Weigang; Wu Bingquan Department of Pathology,Beijing Medical University,Beijing 100083[  
BT,a bcnzo(a)pyrenc-induccd human bronchial carcinoma cell line,has been cstablished.To explore themechanism of benzo(a)pyrenc-induced carcinogenesis,p53 gene mutation in BT was examined. It was found that p53 proteinwas overexpres5ed immunohistochenucally within the nuclci. Abnormal clectrophoresis bands in cxon 7 of p53 gene were de-tected by nonisotopic PCR-SSCP,suggesting a gene mutation. DNA scqucncing showed a transversion of CGG to CTT atcodon 248, which catiscs the changc of the encoded amino acid from arginine to lcucin. The results suggcsted that the p53gene mutation might be involved in the benzo(a)pvene-induccd carcinogcnesis.
【Key Words】: benzo(a)pyrene. lung cancer.p53 gene PCR
【Fund】: 国家自然科学基金
【CateGory Index】: R734.102
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 LIU Yu-hong1,YU Xiao-ying2,HAN Ning3(1. School of Architecture and Civil Engineering,Inner Mongolia University of Technology,Huhhot 010051,PRC;2. Heilongjiang Academy of Environment Science,Harbin 150056,China;3. School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China);THE PRESENT STUDY ON THE TOXIC EFFECT OF BENZO(a) PYRENE (BAP) AND ITS MECHANISM[J];Journal of Inner Mongolia Agricultural University(Natural Science Edition);2008-01
2 Zhang Jun, Zheng Jie, Fang Weigang, et al. Department of Pathology, School of Basic Medical Sciences, Beijing Medical University, Beijing 100083;Correlation between p53 gene mutation and protein expression in 30 cases of human lung cancer[J];NATIONAL MEDICAL JOURNAL OF CHINA;1998-11
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yu Mingkun ; Zhu Cheng; Zhang Guangji(Department of Neurosurgery, Changzheng Hospital,Second Military Medical University,Shanghai,200003 );The changes of excitatory/inhibitory amino acids contents of cerebrospinal flu id and its clinical significance in patients with acute craniocerebral injury[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-02
2 TU Bai\|jie,CHEN Sheng,XIAO Cheng\|feng,et al (Institute of Occupational Medicine,Tongji Medical College, Huazhong University of Science & Technology,Wuhan 430030 China);Study on Neurotoxicity of Benzopyrene in Mice by Microscope,Electron-microscope and Behavioral Methods[J];Industrial Health and Occupational Diseases;2002-02
3 LENG Shu guang,ZHENG Yu xin,LI Xiao hua,et al (National Institute for Occupational Health & Poison Control, Chinese Center for Disease Control & Prevention,Beijing 100050 China);Study on the Relationship between External Exposure Categories and Urinary 1-Hydroxypyrene Concentrations in Coke Oven Workers[J];Industrial Health and Occupational Diseases;2003-05
4 Chen Jiguang et al(Anhui Med.Univ.,21,Meishan Rd.,Hefei,Anhui,China 230032);STUDY ON RELATIONSHIP BETWEEN MANGANESE EXPOSURE AND THE NEUROBEHAVIORAL CORE TEST BATTERY[J];INDUSTRIAL HEALTH AND OCCUPATIONAL OISEASES;1995-04
5 Sun Hong wen Li shuxia (Department of Environmental Science,Nankai University,Tianjin 300071);PHOTOTOXICITY OF POLYCYCLIC AROMETIC HYDROCARBONS[J];;1998-06
6 GONG Zong\|qiang 1,LI Pei\|jun 1,WANG Xin 2;ZHANG Hai\|rong\+1,ZHANG Chun\|gui\+1,Xu Hua\|xia\+1 (1. Institute of Applied Ecology,Chinese Academy of Sciences, Shenyang 110016,China; 2. School of Science,Shenyang University of Technology,Shenyang 11;Cometabolic Degradation of Benzo[a]pyrene in the Soil by the Introduced Fungi[J];Research of Environmental Sciences;2001-06
7 WANG Chong gang 1, YU Qun 2, YU Ang 2, CHEN Rong 2, ZHENG Wei yun 2 (1. School of Life Sience,Xiamen University, Xiamen 361005,China; 2 Environmental Science Research Center, Xiamen University, Xiamen 361005,China);Effect of benzo(a)pyrene and pyrene exposure on hepatic superoxide dismutase in Mugil so-iuy[J];Marine Environmental Science;2002-04
8 XU Nan 1, Wang Chun xia 1, HU Shi bin 2, XU Ying 3 (1 State Key laboratory of Environment Aquatic Chemistry,Research Center for Eco Environmental Sciences,Chinese Academy of Sciences, Beijing 100085,China; 2 Basic Science Department of North W;The induction of aryl hydrocarbon hydroxylase(AHH) of carp liver microsome by five aromatic hydrocarbon compounds[J];Environmental Monitoring In China;2000-06
9 YU Lan,WU Qi,GUAN Ying,et al.Department of Neurology,Baoshan District Renhe Hospital,Shanghai 200431,China;Changes of excitatory amino acids of CSF in patients with acute cerebral infarction and its clinical significance[J];Journal of Clinical Neurology;2005-05
10 LU Wen-Qing 1 ,Richard Gminski 2 ,Volker Mersch-Sundermann 2 (1.Department of Occupational and Environmental Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,China;2.Department of Indoor and Environmental Toxicology,University of Giessen,Giessen D-35385Germany);A Study of Effect of Musk Ketone on Benzo(a) pyrene-induced DNA Damage by Comet Assay[J];Journal of Labour Medicine;2004-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 PAN Zhi zhong, WAN De sen, ZHANG Chang qing, et al.1 Department of Abdominal Surgery of Cancer Center of Sun Yat sen University ofMedical Siences,Guangzhou 510060,P.R. China2 Laboratry Center of Cancer Hospital of Sun Yat sen University of M;Expression of p53,PCNA in colorectal cancer and its clinopathologicalcorrelation and prognostic significance[J];CHINESE JOURNAL OF CANCER;1999-05
2 ZHENG Jie, FANG Wei Gang, WU Bing Quan (Department of Pathology, Peking University School of Basical Sciences, Beijing 100083, China);Biological characteristics of metastatic cancer cells and cloning of human cancer metastasis-related genes[J];Journal of Beijing Medical University;2001-05
3 Zhang Xiangsheng,Zhang Jinling (Department of Pathology,Binzhou Medical College,Binzhou 256603);REALTIONSHIP OF p185,CYCLIND1,RB,p53,BCL-2 GENE PRODUCTS TO THE BIOLOGICALBEHAVIOR OF BREAST CARCINOMA AND THE EXPRESSION OF ER[J];JOURNAL OF BINZHOU MEDICAL COLLEGE;2000-01
4 Zhang Xiangsheng,Zhang Yan,Wu Shuhua, et al (Department of Pathology,Binzhou Medical College Binzhou,Shandong 256603);RELATIONSHIP BETWEEN APOPTOSIS AND THE EXPRESSION OF ER,p53,AND Bcl-2 GENE PRODUCTS AND BIOLOGICAL BEHAVIOR OF BREAST CARCINOMA[J];JOURNAL OF BINZHOU MEDICAL COLLEGE;2000-05
5 Luo Qi,Zhang Sonen,Zhang Shifeng.Department of General Surgery, Xiamen University Zhongshan Hospital, Xiamen 361004, Fujian Province, China;The significance of combined detection of ER,PR,P53 gene and DNA ploidy analysis to prognostic judgment of colorectal cancer[J];Journal of Colorectal & Anal Surgery;2008-03
6 JIA Rui-ping, WEI Jian-ping, ZHANG Xiao-qin,et al. Medical Department, Central Hospital of Baotou City, Neimenggu 014040, China;The Expression and Significant of P73, P53, P21~(waf1) and PCNA Protein in the Different Diseases of Gastric Mucosa[J];Journal of Chinese Physician;2005-S1
7 Wang Lujian,Sun Limei~(*1),Dong Chunling,Sun Limin(Department of Human Anatomy,~(1)Department of Pathology,China Medical University,Shenyang 110001,China);PROGNOSTIC SIGNIFICANCE OF EXPRESSIONS OF APOPTOSIS,P53 AND Bcl-2 IN NON-SMALL CELL LUNG CANCER[J];Chinese Journal of Histochemistry and Cytochemistry;2006-02
8 Zhao Min; Liu Mingqiang; Wang Shizhen; et al. (Dept. of pathol. Second Hospital of HeFei. HeFei 230011);EXPRESSION OF P~(53) AND P~(185) PROTEW IN GALLBLADDER CANCER AND IT'S CLINICAL SIGNIFICANCE[J];HAINAN MEDICAL JOURNAL;2000-04
9 YANG Xue-lian, ZHANG Zhen-xiang, SONG Hui, et al. Insitute for Environmontal Health and Related Product Satety, Chinese Center for Disease Control and Prevention, Beijing 100021, China;DNA Damage and Expression of P53, P21 Protein Induced by N-ethyl-N-nitrosourea in the Primary Cultured Astrocytes of Rats[J];Journal of Environment and Health;2006-01
10 Xu Hong~1,Zhang Guangzheng~2 (1 Henan Tumor Institute,Zhengzhou 450008, China; 2 Medical Experimential Center, Zhengzhou University, Zhengzhou 450052,China);Expression and Clinical Implication of Protein p53 and PCNA in Different Stages of Gastric Carcinogensis[J];Henan Journal of Oncology;2005-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved