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《Journal of Practical Stomatology》 2003-04
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The effects of enamel matrix proteins on the synthesis of alkaline phosphatase and type I collagen in osteoblasts

Shu Rong,Wu Chunlan.Department of Oral Medicine,Ninth People's Hospital,Shanghai Second Medical University,Shanghai 200011  
Objective:To study the effects of enamel matrix proteins (EMPs) on the biological characters of osteoblasts.Methods:MC3T3-E1 osteoblasts were cultured in vitro and exposed to EMPs at 300,400 and 500 μg/ml.Enzyme kinetic method was used to assay alkaline phosphatase (ALP) synthesis and 3H-proline labelling combining collagenase digestion method was used to assay type I collagen synthesis.Results:The results manifested that ALP level in EMPs treated groups was higher than that in control group (P0.01) .The synthesis of type I collagen in EMPs treated groups was signifiantly increased (P0.01). Conclusions: EMPs may stimulate the function of osteoblasts and may play an important role in the regeneration of alveolar bone.
【CateGory Index】: R780.2
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