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《Journal of Practical Stomatology》 2005-05
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Effect of static magnetic field on the proliferation and differentiation of rat osteoblasts

Qiu Lihong, Bao Yang, Zhong Ming, et al. College of Stomatology, China Medical University, Shenyang 110002,China  
Objective:To Study the effect of static magnetic field on the proliferation and differentiation of osteoblasts.Methods:In vitro cultured rat calvarial osteoblasts were seeded into 96-well culture plate and exposed to the different magnetic intensity field for 24,48 and 72 h respectively.MTT assay was applied to study the cell proliferation. ALP was measured by a microplate reader.Results:When the magnetic exposure time extended to 48 h or 72 h, the corresponding MTT value of osteoblasts with magnetic treatment of 40 mT or 62 mT increased obviously(P0.05), but such proliferative effect was not shown in the 108 mT treatment group (P0.05). ALP activity increased after 24 h exposure to static magnetic field, the effect of static magnetic field intensity of 62 mT and 83 mT showed the strongest promotion effect(P0.05).Conclusion:Within a certain range of magnetic intensity, magnetic field enhances osteoblasts growth and the alkaline phosphatase activity.
【Key Words】： Static magnetic field Osteoblast cell Cell proliferation Differentiation
【Fund】： 辽宁省教育厅基金资助 项目编号:20122170
【CateGory Index】： Q689;
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