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《Journal of Practical Stomatology》 2008-03
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Effect of static magnetic field on intracellular calcium concentration of osteoblast cell

ZHU Linlin,QIU Lihong. Department of Endodontics,College of Stomatology,Chinese Medical University, Shenyang 110002, China  
Objective:To observe the proliferation and the free intracellular calcium ion concentration of osteoblast under the effect of static magnetic field and discuss the possible mechanism of the static magnetic field on promoted the proliferative function. Methods: The MTT assay was used to measure the rate of the proliferation on different magnetic field intensity(0, 0.026, 0.044, 0.090 T)and action time(24, 48, 72 h).The fluorescence double wavelength spectrophotometer was used to measure the concentration of intracellular calcium ion. The data obtained were analyzed for ANOVA and t-test using SPSS 11.0 software package. Results: Osteoblasts in group 0.044 T appeared significant proliferation within 48 h(P0.01).When the static magnetic field effected on the cell at 72 h, the group 0.044 T and group 0.090 T were rising significantly comparing with group 0 T(P0.01).The [Ca2+]i of osteoblast in the group 15 min was increased significantly under the static magnetic field of 0.044 T.Conclusion:The static magnetic field can accelerate the proliferation of the cultured osteoblast in the intensity and time dependent way. Certain intensity of static magnetic field can increase the concentration of the intracellular calcium ion,and may be dependent on time.
【Key Words】： Static magnetic field Osteoblast Proliferation Concentration of the intracellular calcium ion
【CateGory Index】： Q27
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