Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update

Measurement of ALPase activity of cultured osteoblasts

Li Dehua, Liu Baolin, Wu Junzheng, et al. Dept. of Oral Maxillofacial Surgery, College of Stomatology, The Fourth Military Medical University, Xi'an 710032  
The intercellular ALPase activity is a key criterion for evaluating the differentiation and the function of osteoblast in vitro . On the basis of the principle mechanism of ALPase activity analysis, the authors modified the method of ALPase activity measurement of the cultured osteoblasts. The cells were cultured in 96 well plates and lyzed with 200μl of 0.05% Triton X 100 associated with supersonization. The reaction of ALPase with the substrate was stopped by 0.4mol/L of NaOH. The ratio of 40∶100∶100 of lysate to substrate and to 0.4 mol/L of NaOH showed better sensitivity of the assay and reliability of the results
【CateGory Index】: R446.112
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 JIANG Ying1,WANG Xiang2,XU Hua1,LIU Ying-qun1. 1.Department of Pediatric Dentistry,School of Stomatology,Harbin Medical University,Heilongjiang Harbin 150001,China;2.Department of Planting Stomatology,2nd Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150086,China;Effects icariin of on the alkline phosphatase activity of human periodontal ligament cells inhibited by LPS[J];临床口腔医学杂志;2013-03
2 ZHANG Jin-zhu1,QI Yi-tian,LI Peng-dong2(1.Department of Neurosurgery,First Hospital,Jilin University,Changchun 130021,China; 2.Department of Tissue Engineering,School of Pharmacy,Jilin University,Changchun 130021,China);Morphology and function detection of chondrocytes differentiated from bone marrow mesenchymal stem cells induced by supernatant of chondrocytes[J];吉林大学学报(医学版);2012-03
3 WANG Yuan-sheng1,2,LI Fa-di1,3,HAO Zheng-li1,YU Lian-ping4(1.Faculty of Animal Science and Technology,Gansu Agricutural University,Lanzhou 730070,China;2.Lanzhou Husbandry & Veterinary Institute,Lanzhou 730050,China;3.Gansu Feed Engineering Technology Research Center,Lanzhou 730070,China;4.Gansu Animal Nutrition Institute,Lanzhou 730046,China);Effects of Trifolium pratense cv 'Minshan' isoflavone on performance and bone mineralization and blood profile of laying hens in later period[J];草业学报;2010-02
4 CHEN Zhu-su, ZHOU Lei, WU Wang-xi. Guangdong Provincial Stomatological Hospital & the Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510280, China;Effects of Polypeptide P-15 on Attachment,Proliferation and Differentiation of Cultured Osteoblast on Bone Mineral Particles[J];广东牙病防治;2010-04
5 PANG Nan,ZOU Jing-cai,XUN Wen-xing,ZHANG Hong-mei,WANG Yan,QU Xiao-li,ZHANG Xiu-li,YANG Hai-zhen,YANG Xiang-ju,CHEN Jing-tao,CHEN Xiao-nan(Department of stomatology,Tang Du Hospital,The Fourth Military Medical University,Xi'an 710038,China);Differentiation and mineralization-inducing effect of polyvinyl alcohol on rat dental pulp cells[J];牙体牙髓牙周病学杂志;2010-01
6 He Kang,et al(Laboratory of Physiological Science,Guangdong Medical College,Zhanjiang 524023);Prediction of the Marrow Stromal Cells with Grey Model[J];数理医药学杂志;2007-05
7 YU Feng~1 HONG Peng-zhi~1 YANG Ping~1 ZENG Shao-kui~1 CUI Liao~2 AI Chun-mei~2 (1 School of Food Science and Technology,Guangdong Ocean University,Zhanjiang,Guangdong 524025; 2 Guangdong Medical College,Zhanjiang,Guangdong 524023);Effects of Enzymatic Hydrolysate from Tilapia Skin on Bone Marrow Stromal Cells of Rats in Vitro Culture[J];现代生物医学进展;2007-09
8 Liu YY, Cui L, Xu BL, Yao WM 1Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China; 2Institute of Respiratory Disease, Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China;Prediction of in vitro cultured osteoblast with a grey dynamic model[J];中国组织工程研究与临床康复;2007-37
9 Hu Xiao-yi1, Luo Li2, 1Comprehensive Outpatient Department, 2De-partment of Oral and Maxillofacial Surgery, Hospital of Stomatology,Guangxi Medical University, Nanning 530021, Guangxi Zhuang Au-tonomous Region, China;Influence of discontinuous pressure on the proliferation and differentiation of in vitro cultured adult bone marrow stromal cells[J];中国临床康复;2005-46
10 CHEN Xu,YANG Zhiming,XIE Huiqi,et al.Department of Orthopaedics,Yantai-Mountain Hospital of Yantai,Yantai Shandong,264000,P.R.China;BIOLOGICAL EFFECT OF WO-1 ON HUMAN EMBRYONIC OSTEOBLASTS[J];中国修复重建外科杂志;2005-10
Chinese Journal Full-text Database 10 Hits
1 WEN Yi-fei1,CAO Guo-jun1,FAN Jiang-wen2,MAO Hua-ming1,LUO Fu-cheng1(1.Faculty of Animal Science,Yunnan Agriculture University,Kunming 650201,China;2.Institute of Geographical Sciences and Natural Resources Research,the Chinese Academy of Sciences,Beijing 100101,China);Relationship between phenols and in vitro digestibility of six legume feeding shrubs[J];草业学报;2009-01
2 DU Zhan-chi,FAN Jiang-wen,ZHONG Hua-ping(Institute of Geographical Science and Natural Resources,Chinese Academy of Sciences,Beijing 100101,China);Biological absorbing ability of chemical elements in Trifolium pratense and Dactylis glomerata[J];草业学报;2008-02
3 LI Xiao-kun1,LU Jian-wei1,CHEN Fang2(1.College of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,China;2.Botanical Garden of Wuhan,Wuhan 430074,China);Primary study on fertilizer application of forage[J];草业学报;2008-02
4 CHEN Ben-jian(College of Grassland Science,Gansu Agicultural University,Lanzhou 730070,China);Legume forage resources and floristic characteristics in Gansu Province[J];草业科学;2008-04
5 ZHANG Yong1,ZHU Yu-jing1,REN Hui-ling2,SUN Jing-xia3,WANG Rui4(1.College of Veterinary and Animal Science,Shenyang Agricultural University,Shenyang 110161,China; 2.Division of Animal Science,Liaoning Vocational College,Tieling Liaoning 112001,China; 3.Bird-singing Woods,Dalian Laohutan Ocean Park,Dalian Liaoning 116013,China; 4.Fushun Animal Diseases Prevention Center in Liaoning Province,Fushun Liaoning 113006,China);Effects of Isoflavone Extract from Red Clover on Growth Performance and Immune Function in Mice[J];沈阳农业大学学报;2008-01
6 ZHANG Sun,GE Lihong. Peking University School and Hospital of Stomatology, Beijing 100081;Analysis of related factors effecting on the root apical type of immature permanent teeth after apexification[J];现代口腔医学杂志;2007-04
7 LIU Yuan-yuan,MIAO Chun-lei,TANG Sheng-jian Department of Plastic Surgery,Weifang Medical College,Weifang 261042,China;Study of chondrocytic differentiation of bone marrow stem cells with the supernatant of chondrocytes in vitro[J];细胞与分子免疫学杂志;2007-07
8 LIN Kun,ZHU Bi-liu,ZENG Yang,et al.Department of Public Health,Shantou University Medical College,Shantou 515041,China;Evaluation on statistical and grey models to predict daily SARS cases[J];华南预防医学;2007-02
9 Zhang Shu,Zhang Qiang,Yin Zhi-cheng.(School of Public Health,Sichuan University,Chengdu 610041,China.);THE APPLICATION OF GREY MODEL(1,1) IN THE PREDICTION OF SCHISTOSOMIASIS INFECTION RATE[J];现代预防医学;2007-07
10 YANG Bao-hua 1,Wang Xiao-ping 2,Chen Yong-gan3(1.Higher Mathematics Department of Pharmacy College,Henan University,Henan Kaifeng 475001,China;2.Mathematics Department of Xuechang College, Henan Xuchang 461000,China;3.Pharmacy Specialty Laboratory of Pharmacy College,Henan University,Henan Kaifeng 475001,China);Gray mathematics model of sustained release of Nimoldipine Slow-released Tablets[J];河南大学学报(医学版);2006-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 You Ying;Han Chun-yan;Chaudhry Maria Tabassum;Li Ling;Yao Jia-ying;Wang Sheng-nan;Yang Jia-xin;Teng Nan;Li Yao;College of Animal Sciences and Technology,Northeast Agricultural University;;Effect of Quercetin on Egg Quality and Components in Laying Hens of Different Weeks[J];Journal of Northeast Agricultural University(English Edition);2015-04
2 XU Lili;CHEN Xiuying;LIU Zhenhua;Zhongguancun Hospital;;Research of effects of the IL-6 activity of human periodontal ligament cells on treating with icariin inhibited by LPS[J];现代口腔医学杂志;2015-02
3 Lin Yang-dong;Zhao Bo;Yin Kai;First Clinical College of Tianjin Medical University;Department of Stomatology, First Central Hospital of Tianjin;;Effect of basic fibroblast growth factor on human periodontal ligament cells cultured in vitro[J];中国组织工程研究;2014-20
4 ZHANG Yun-xia;LIU-lu;YU Ming-hang;XIE Jin-ge;ZENG Qiang-cheng;School of Life Science,Shandong Normal University;Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics,Institute of Biophysics,Dezhou University;School of Life Science,Dezhou University;;Research on the Effects of Mechanical Stimulation on the Proliferation and Differentiation of Stem Cell[J];德州学院学报;2014-02
5 PANG Ting-ting;CUI Liao;LIU Yu-yu;ZENG Tao;Zhanjiang Central Blood Station;;Effect of tanshinol and salvianolic acid B on osteogenesis and adipogenesis of marrow stroma stem cells[J];广东医学院学报;2014-01
6 Sheng Huagang;Pharmacy School of Shandong University of Traditional Chinese Medicine;;Advances in Research of Icariin on Cell Proliferation[J];化工时刊;2013-08
7 YU Lian-ping1,2,HAO Zheng-li1,LI Fa-di1,3,MENG Xiang-jun2,CHEN Xing-rong2,LI Xin-yuan2(1.Key Laboratory of Grassland Ecosystem of Ministry of Education,Faculty of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Animal Nutrition Institute,Lanzhou 730010,China;3.Gansu Feed Engineering Technology Research Center,Lanzhou 730070,China);Effect of isoflavone in red clover on the growth and immune functions, and in the antioxidant indices in ovariectomized rats[J];草业学报;2012-06
8 Deng Lei, Yu Zhan-hai, Yin Li-hua, Wu Xi-xi School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China;Effects of carboxymethyl chitosan and platelet-derived growth factor-BB on proliferation of human periodontal ligament cells cultured in vitro[J];中国组织工程研究;2012-51
9 ZHU Yujing1 PAN Xiangcen1 ZHANG Yong1 LI Xinwei1 MA Yong1 SHAO Caimei2(1.College of Animal Science and Veterinary,Shenyang Agricultural University,Shenyang 110866,China;2.Liaoning Well Hope Agri-Tech Co.Ltd.,Shenyang 110164,China);Red Clover Isoflavones:Extraction Methods and Physiological Function[J];动物营养学报;2012-12
10 JIANG Yibao1 YANG Yurong1 CUI Guowen2 WANG Chenzhang1(1.College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China; 2.College of Animal Science,Northeast Agricultural University,Haerbin 150030,China);Effects of Red Clover(Trifolium pratense) Isoflavones on Several Blood Endogenous Hormone Levels of Broilers[J];动物营养学报;2012-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved