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《JOURNAL OF PRACTICAL STOMATOLOGY》 1997-01
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Measurement of ALPase activity of cultured osteoblasts

Li Dehua, Liu Baolin, Wu Junzheng, et al. Dept. of Oral Maxillofacial Surgery, College of Stomatology, The Fourth Military Medical University, Xi'an 710032  
The intercellular ALPase activity is a key criterion for evaluating the differentiation and the function of osteoblast in vitro . On the basis of the principle mechanism of ALPase activity analysis, the authors modified the method of ALPase activity measurement of the cultured osteoblasts. The cells were cultured in 96 well plates and lyzed with 200μl of 0.05% Triton X 100 associated with supersonization. The reaction of ALPase with the substrate was stopped by 0.4mol/L of NaOH. The ratio of 40∶100∶100 of lysate to substrate and to 0.4 mol/L of NaOH showed better sensitivity of the assay and reliability of the results
【CateGory Index】: R446.112
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