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《Journal of Clinical and Experimental Medicine》 2017-06
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Effects of gastrin in the proliferation of human gastric cancer cell MKN45 and expression of osteopontin

CHEN Qing-fa;XU Yan;XUE Fang-xi;Department of Gastroenterology,Yishui Central Hospital of Linyi;  
Objective To explore the effect of gastrin in the proliferation of human gastric cancer cell MKN45 and expression of osteopontin. Methods Drug intervention groups were divided into three groups,gastrin group( 10~(-6) mol / L),gastrin( 10~(-6)mol / L) + proglumid group( 10~(-2)mol / L),the control group without drug culture liquid. They were cultured for 24 h,48 h. After the end of the culture supernatant,microplate assay was used to determine optical density value for 3 times. The expression of OPN was detected by radioimmunoassay( RIA) and the proliferation of human gastric cancer cell line MKN45 was detected by MTT assay. Results The gastrin group had more promotion impact on the proliferation of human gastric cancer cells MKN45 compared with the control and combination group with significant difference( P 0. 05). OPN were detected in the gastric cancer cell line MKN45 culture solution,the increase of expression was related with the culture time. The proliferation of human gastric cancer cell line MKN45 the and expression of OPN was increased in the gastrin group( P 0. 05). Conclusion Gastrin can promote the proliferation of human gastric cancer cell line MKN45 and promote the expression of OPN,suggesting that gastrin promotes the expression of MKN45 in gastric cancer cells and has a certain relationship with the expression of OPN. And its receptor antagonist proglumide may play a significant role in inhibiting this promoting mechanism.
【CateGory Index】: R735.2
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