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《Chinese Journal of Practical Internal Medicine》 2006-24
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Curcumin inhibits the activation marker of hepatic stellate cells by up-regulating the peroxisome proliferator-activated receptorγ.

Cheng Yang,Ping Jian,Xu Lieming,et al.Institute of Liver Diseases,Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China  
Objective To study the relation between the effect of Curcumin on the proliferation and activation of hepatic stellate cells(HSC)and the expression of the peroxisome proliferator-activated receptorγ(PPARγ).Methods The rat HSC was isolated from SD rats through in situ perfusion of liver with Pronase E and density-gradient centrifugation with Nycodenz.The subcultured cells were treated with corresponding compound.The inhibition effect on HSC proliferation was determined by MTT colorimetry.The total RNA was extracted by TRizol reagent,and the gene expression level of PPARα and smooth muscle actin (α-SMA)were determined by semi-quantitative RT-PCR.The total cellular proteins were extracted and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis,and the protein level of α-SMA was determined by Western blotting.Results The MTT analysis results showed that Curcumin inhibited HSC proliferation between 10 and 50 μmol/L in a dose-dependent manner.In the Day 1,4,7 of primary HSC and passaged HSC,the expression of PPARγ mRNA decreased with HSC activation extent;Curcumin could up-regulate the expression level of PPARγ.Curcumin suppressed the expression of α-SMA not only at the gene level but also at the translation level.But all these effects of Curcumin on HSC could be blocked by the PPARγ specific antagonist GW9662.Conclusion The effect of Curcumin on HSC cell proliferation and activation is through the up-regulation of PPARγ signal.
【Fund】: 国家自然科学基金(30300458)
【CateGory Index】: R285.5
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Chinese Journal Full-text Database 3 Hits
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