Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Practical Internal Medicine》 2006-24
Add to Favorite Get Latest Update

Curcumin inhibits the activation marker of hepatic stellate cells by up-regulating the peroxisome proliferator-activated receptorγ.

Cheng Yang,Ping Jian,Xu Lieming,et al.Institute of Liver Diseases,Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China  
Objective To study the relation between the effect of Curcumin on the proliferation and activation of hepatic stellate cells(HSC)and the expression of the peroxisome proliferator-activated receptorγ(PPARγ).Methods The rat HSC was isolated from SD rats through in situ perfusion of liver with Pronase E and density-gradient centrifugation with Nycodenz.The subcultured cells were treated with corresponding compound.The inhibition effect on HSC proliferation was determined by MTT colorimetry.The total RNA was extracted by TRizol reagent,and the gene expression level of PPARα and smooth muscle actin (α-SMA)were determined by semi-quantitative RT-PCR.The total cellular proteins were extracted and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis,and the protein level of α-SMA was determined by Western blotting.Results The MTT analysis results showed that Curcumin inhibited HSC proliferation between 10 and 50 μmol/L in a dose-dependent manner.In the Day 1,4,7 of primary HSC and passaged HSC,the expression of PPARγ mRNA decreased with HSC activation extent;Curcumin could up-regulate the expression level of PPARγ.Curcumin suppressed the expression of α-SMA not only at the gene level but also at the translation level.But all these effects of Curcumin on HSC could be blocked by the PPARγ specific antagonist GW9662.Conclusion The effect of Curcumin on HSC cell proliferation and activation is through the up-regulation of PPARγ signal.
【Fund】: 国家自然科学基金(30300458)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Wo Xingde,Hong Xingqiu,Gao Chengxian,et al Molecular Medical Institution,Zhejiang College of TCM,Hangzhou(310009);Long-period Virulent Test of Curcumin[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;2000-01
2 Zhang Hang1 Sun Shoucai2 Song Jian2 Liu Lijun3(1.Dental Hospital of Xianyang City,Shaanxi,712000;2.Shaanxi University of Chinese Medicine,712046;3.Shaanxi Traditional Chinese Medicine Hospital,710000);Clinical Efficacy Observation of Curcumin in the Treatment of Chronic Hepatitis B Hepatic Fibrosis[J];Modern Traditional Chinese Medicine;2007-03
3 SHU Jian-chang,WANG Hong-li,YE Guo-rong,HE Ya-jun,Lu xia,FANG Li(Affiliated Guangzhou Red Cross Hospital of the Medical College of Jinan University,Guangzhou 510220,China);The Study of Therapeutic Effects of Curcumin on Hepatic Fibrosis and Variation of Correlated Cytokine[J];Journal of Chinese Medicinal Materials;2007-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved