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《Journal of Shenyang Agricultural University》 2005-01
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Studies on Protoplast Preparation and Regeneration of Trichoderma spp.T21,T22

ZHAO Le-hui, LI Ying, LU Shu-xia (Shenyang Agricultural University, Shenyang 110161,China)  
This paper reports the influence of mycelic age, enzyme proportion, osmotic stablizer-buffer systems as well as enzymolysis time. By using compound enzyme composed of 5.0g·L-1 cellulase, 2.5g·L-1 snailase, 2.5g·L-1 lywallzyme, osmotic stablizer-buffer systems composed of 0.2mol·L-1 PBS, pH5.8 and 0.6mol·L-1 sucrose, protoplast were obtained after 3-hour treatment of the 20h-old mycelia at 30℃. The number of the protoplasts was about 2.7×109·L-1
【CateGory Index】: S476.1
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