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《Journal of Shenyang Agricultural University》 2008-04
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Determination of α-Solanine in Eggplant Fruit with HPLC

LI Zhi-wen1a,ZHOU Bao-li1a,LIU Xiang2,DING Yu-wen1a,CHANG Lin-lin1a,BAI Li-ping1b (1.a.College of Horticulture,b.College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China;2.College of Agriculture and Bioengineering,Tianjin University,Tianjin 300072,China)  
The high performance liquid chromatography(HPLC) analysis was used in this study to develop a simple and effective quantitative analysis method of the α-solanine in eggplant.The separation effect and retention time under different chromatography conditions were studied to determine the optimal HPLC condition.The results showed that the optimal separation was achieved on a Waters Nova-pak C18 column.The mobile phase was acetonitrile-0.05mol·L-1 potassium dihydrogen phosphate(70:30,V/V,pH 4.5).The floe rate was 0.7mL·min-1 and determination wavelength was set at 205 nm and the column temperature was 25 ℃.The calibration curve was linear in the range of 0.15~3.00g·L-1.The average recovery for α-solanine was 97.97 % and RSD was 1.17%(n=5).This analysis method gave a high accuracy,so this HPLC analysis method is applicable for qualitative and quantitative analysis of α-solanine in eggplant fruit.
【Fund】: 国家“863”计划资助项目(2004AA247010)
【CateGory Index】: S641.1
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