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《Journal of Applied Clinical Pediatrics》 2006-09
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Effect of Conditioned Immune Response on Nicotinic Acetylcholine Receptor and Choline Acetyltransferase Expression in Synovium of Collagen Induced Arthritis Rats

LI Xiu-yun~1,FANG Hong~1,WANG Hong-wei~1,WANG Jin-rong~2,WANG Dan-dan~1,SHI Hong~1(1.Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;2.Department of Pediatrics,Shandong Provincial Hospital,Clinical Medical College of Shandong University,Jinan 250021,China)  
Objective To investigate the expression of the protein and mRNA of the nicotinic acetylcholine receptor α7(nAChRα7),choline acetyltransferase(ChAT) in the rats of conditioned immune response(CIR) on collagen induced arthritis(CIA) and to explore the probable mechanism of effects of CIR on CIA in rats.Methods The rats were divided into normal controls and experimental groups.An experimental arthritis models of healthy Wistar rats [male,4 to 5 weeks old,(150±10) g weight] were established by intradermal injection of Ⅱ type collagen.After that,all rats were divided into 5 groups randomly and treated differently.In group of CIR,the smell of camphor was taken as conditioned stimulus(CS) and MTX+Pred as unconditioned stimulus(UCS),respectively.A conditioned immune response models of rats with CIA were established by the combination of CS and UCS 7 times(7 days),then these rats were given CS only for another 21 days;in group MTX+Pred,these rats were treated with MTX+Pred for 4 weeks;in the group of lower dose of MTX+Pred,rats were treated with MTX+Pred for the first 7 days,then with placebo until 4 weeks;in the group of only smelling camphor,rats received the smell of camphor for 4 weeks;in the group of blank controls,rats were treated with placebo for 4 weeks.Then the protein and mRNA of nAChRα7,ChAT in synovium were detected by immunohistochemical and RT-PCR methods.Results In group of CIR,the expressions of nAChRα7 and ChAT were increased significantly than those of the blank controls group(all P0.01);the expressions of nAChRα7 and ChAT decreased markedly in the blank controls,compared with the normal controls and CIR group(all P0.01).Conclusion A CIR established by the combination of sense of smell and immunosuppressants can up-regulate the expressions of nAChRα7,ChAT in synovium and activate the nicotine cholinergic pathway to reduce the inflammation at last.
【Fund】: 国家自然科学基金项目资助(30571739)
【CateGory Index】: R593.2
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Jin-rong1a,LI Xiu-yun2,WANG Hong-wei2,FAN Hui1b,HAN Xiu-zhen1a,LIU Zhi-gang1a,SUN Yan1a,LI Hua-bing1a(1a.Department of Pediatrics ;1b.Department of Pathology,Shandong Provincial Hospital,Shandong University,Jinan 250021,China;2.Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Mechanisms of Stimulating Vagus Nerve on CD4~+ T Lymphocytes Activation in Experimental Arthritis Rats[J];Journal of Applied Clinical Pediatrics;2007-09
【Citations】
Chinese Journal Full-text Database 6 Hits
1 WANG Jinrong, WANG Hongwei Department of Paediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Inflammatory reflex—cholinergic anti-inflammatory pathway[J];Chinese Journal of Neuromedicine;2004-04
2 WANG Jin-rong*, WANG Hong-wei*, LIU Sheng-hong, TAO De-ding, KANG Min*, WANG Fang, LI Xiu-yun*, SHI Hong*. *Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Inherent cholinergic system in immunocytes and immune organs of rats[J];Chinese Journal of Neuromedicine;2005-01
3 ZHEN Xiao-fang,MA Song-chun,YAO Yuan(Beijing Children′s Hospital of Capital Medical University,Beijing 100045,Chin a);Study on prognosis of 96 cases of children with juvenile rheumatoid a rthritis[J];Journal of Applied Clinical Pediatrics;2004-03
4 WANG Jin-rong 1,HAN Xiu-zhen 1, WANG Yong-kang 2,LV Xiao-xia 3,DU De-li 2,HAN Bo 1,ZHUANG Tian-xin 1,LI Qiu-bo 1,MA Pei-ran 1 (1.Department of Pediatrics;2.Department of Pathology;3.Central Lab,Shandong Provincial Hospital, Clinical Medical College of Shandong University, Jinan 250021, China);Expression of vascular endothelial growth factor in synovium of rats with adjuvant arthritis[J];Journal of Applied Clinical Pediatrics;2004-05
5 Huang Jingxin, Lin Wenjuan, Chen Jihuan (Brain-Behavior Research Center, Institute of Psychology, Chinese Academy of Sciences, Beijing,China 100101);RESEARCH PROGRESS IN CONDITIONED IMMUNOENHANCEMENT[J];Journal of Chinese Psychology Acta Psychologica Sinica;2003-01
6 SHU Jian-Xiang,WANG Jin-Rong,WANG Hong-Wei,CHENG Pei-Xuan,SHI Hong,KANG Min, HU Xiu-Fen.Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huaz hong University of Science and Technology,Wuhan 430030,China [;The curative effect of conditioned immune response on collagen induced arthritis in rats[J];Chinese Journal of Immunology;2004-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Jin-rong*, WANG Hong-wei*, LIU Sheng-hong, TAO De-ding, KANG Min*, WANG Fang, LI Xiu-yun*, SHI Hong*. *Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Inherent cholinergic system in immunocytes and immune organs of rats[J];Chinese Journal of Neuromedicine;2005-01
2 HUANG Fan, YANG Jing, ZHONG Fei, XU Xue, MA Zhong-fu (Department of Neurology, the First Hospital of Sun Yat-sen University, Guangzhou 510080, China);Clinical Significance of Measuring the Activities of Serum Cholinesterase(CHE) in Patients with Acute Massive Cerebral Infarction[J];Journal of Tropical Medicine;2008-10
3 WANG Jin-rong 1,HAN Xiu-zhen 1, WANG Yong-kang 2,LV Xiao-xia 3,DU De-li 2,HAN Bo 1,ZHUANG Tian-xin 1,LI Qiu-bo 1,MA Pei-ran 1 (1.Department of Pediatrics;2.Department of Pathology;3.Central Lab,Shandong Provincial Hospital, Clinical Medical College of Shandong University, Jinan 250021, China);Expression of vascular endothelial growth factor in synovium of rats with adjuvant arthritis[J];Journal of Applied Clinical Pediatrics;2004-05
4 CHEN Fang,LI Zi-jiang,LIU Jing (Beijing Childrens′ Hospital Attached to Capital University of Medical Science,Beijing 100045,China);Clinical Analysis of 10 Cases of Juvenile Rheumatoid Arthritis Associated Iridocyclitis[J];Journal of Applied Clinical Pediatrics;2005-07
5 YANG Yang~1,WANG Dan-dan~1,WANG Hong-wei~1,HU Xian-ming~2,CHENG Pei-xuan~1(1.Department of Pediatrics,the Affiliated Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030,China;2.Sanitary Bureau in Wuchang, Wuhan 430060, China);Effect of Juvenile Rheumatoid Arthritis Active Serum on Cell Cycle,Apoptosis of Dendritic Cell and Cell Proli-feration of Splenic Lymphocytes[J];Journal of Applied Clinical Pediatrics;2005-08
6 MIN Yue, XU Qin - ying, WANG Li - feng, SONG Xiao - xiang, NJ Zheng, CHENG Jiang, FENG Qi - hua , LI Xiao -zhong (Kidney Department of Children's Hospital Afficiated to Soochow University,Suzhou 215003,China);Clinical Study in 40 Children with Systemic Juvenile Idiopathic Arthritis[J];Journal of Applied Clinical Pediatrics;2005-11
7 XU Po - ski, WANG Xia, HU Hong, XU Jin ( Medical Laboratory Center; Department of Pediatrics,Henan Provincial People s Hospital, Zhengzhou 450003,China);Significance of Anticyclic Citrullinated Peptide Antibody in Juvenile Idiopathic Arthritis[J];Journal of Applied Clinical Pediatrics;2005-11
8 MAO Hai-ying,CAO Lan-fang,MA Min,ZHAO Yu(Department of Pediatrics,Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University,Shanghai 200001,China);Thirty-Seven Cases with Juvenile Idiopathic Arthritis[J];Journal of Applied Clinical Pediatrics;2006-09
9 WANG Jin-rong1a,LI Xiu-yun2,WANG Hong-wei2,FAN Hui1b,HAN Xiu-zhen1a,LIU Zhi-gang1a,SUN Yan1a,LI Hua-bing1a(1a.Department of Pediatrics ;1b.Department of Pathology,Shandong Provincial Hospital,Shandong University,Jinan 250021,China;2.Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Mechanisms of Stimulating Vagus Nerve on CD4~+ T Lymphocytes Activation in Experimental Arthritis Rats[J];Journal of Applied Clinical Pediatrics;2007-09
10 LIU Zhi-gang1,WANG Xue-yu2,CHEN Lu-yuan3,HAN Xiu-zhen2,FENG Yi-zhen2,SUN Yan2,LI Hua-bing2,WANG Jin-rong2(1.Department of Pediatrics,Maternal and Child Health Hospital of Jinan,Jinan 250001,Shandong Province,China;2.Department of Pediatrics,Shandong Provincial Hospital,Jinan 250021,Shandong Province,China;3.Department of Pediatrics,Shandong Rongjun Hospital,Jinan 250010,Shandong Province,China);Expression of Neuronal Acetylcholine Receptor Alpha 7 on CD4~+ T Lymphocytes of Peripheral Blood in Children with Asthma[J];Journal of Applied Clinical Pediatrics;2008-16
【Co-references】
Chinese Journal Full-text Database 6 Hits
1 WANG Jinrong, WANG Hongwei Department of Paediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Inflammatory reflex—cholinergic anti-inflammatory pathway[J];Chinese Journal of Neuromedicine;2004-04
2 WANG Jin-rong*, WANG Hong-wei*, LIU Sheng-hong, TAO De-ding, KANG Min*, WANG Fang, LI Xiu-yun*, SHI Hong*. *Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Inherent cholinergic system in immunocytes and immune organs of rats[J];Chinese Journal of Neuromedicine;2005-01
3 Wang Keyi, Yang Chun, Leng Jianhang, et al. Department of Nuclear Medicine, Hangzhou First People’s Hospital, Hangzhou (310006);Expression of Activation Markers on Lymphocytes in Synovial Fluid From Patients with Rheumatoid Arthritis[J];Journal of Radioimmunology;2005-04
4 LI Juan, CHEN Hong-Zhuan (Department of Pharmacology, Shanghai Second Medical University, Shanghai 200025);The Non-Neuronal Cholinergic System in Immunocompetent Cells[J];Progress In Physiological Sciences;2005-03
5 Wang Jinrong 1,Wang Hongwei 1,Liu Shenghong 2 et al 1Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 2Department of Anatomy ,School of Basic Medical Sciences,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030;Cholinergic System in Peripheral Blood CD4+T Lymphocytes of Rats with Collagen-induced Arthritis[J];Acta Universitatis Medictnae Tangji;2005-01
6 ZHANG Ling Ling, SHEN Yu Xian, WEI Wei (Institute of Clinical Pharmacology,Anhui Medical University, Hefei 230032);Relation between animal models of RA and clinic[J];Chinese Pharmacological Bulletin;2002-05
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 WANG Jinrong, WANG Hongwei Department of Paediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Inflammatory reflex—cholinergic anti-inflammatory pathway[J];Chinese Journal of Neuromedicine;2004-04
2 ZHANG Jianglin,HUANG Feng.Department of Rheumatology,PLA General Hospital,Beijing 100853,China;Clinical characters of juvenile onset spondyloarthropathies[J];Chinese Journal of Rheumatology;2001-02
3 ZHEN Xiao-fang,MA Song-chun,YAO Yuan(Beijing Children′s Hospital of Capital Medical University,Beijing 100045,Chin a);Study on prognosis of 96 cases of children with juvenile rheumatoid a rthritis[J];Journal of Applied Clinical Pediatrics;2004-03
4 Li Bo Lin Wenjuan (Brain-Behavior Research Center Institute of Psychology Chinese Academy of Sciences, Beijing 100012,China);STUDIES ON ANIMAL'S CONDITIONED IMMUNE MODULATION[J];Journal of Chinese Psychology Acta Psychologica Sinica;1996-03
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