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《Journal of Applied Clinical Pediatrics》 2006-18
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Comparison of Microbial Community Structure in Rotavirus Infectious and Healthy Children

DU Hui-min~1,CHEN Ying-hong~1,CHEN Jun-yi~1,ZHANG Mei-ling~2,ZHAO Li-ping~2,PANG Xiao-yan~2(1.Department of Pediatrics,Xinhua Hospital,Medical School of Shanghai Jiaotong University,Shanghai 200092,China;2.Laboratory of Molecular Microbial Ecology and Ecogenomics,Shanghai Jiaotong University,Shanghai 200240,China)  
Objective To compare the composition of microbial community in rotavirus infectious children and that in healthy children.Methods Fecal samples were selected from 20 rotavirus infectious children and 6 healthy children.The total genomic DNA of bacteria was extracted from each sample.Molecular hybridization was performed with a mixed probe from the labeled enterbacterial repecitive intergenic consensus(ERIC)-polymerase chain reaction(PCR) product of one sample,then PCR-temperature gradient gelelec-trophoresis(TGGE) analysis of amplified 16S rDNA V3 regions was carried out.Results High GC-bacteria was less in rotavirus infectious children than that in healthy children.Host specific feature was found in intestinal microflora.Conclusions Rotavirus infection can cause the disorder of the gut microflora.Antibiotic treatment and probiotic treatment can affect the recover of gut flora differently.The balance of microbial community can be interrupted by antibiotic treatment,while probiotic is helpful for the recovery.Some information obtain will be valuable for clinical application.It is indicated that antibiotie will be used cautiously in child with rotavirus infection.
【Fund】: 国家自然科学基金项目资助(30370031)
【CateGory Index】: R725.1
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