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《Journal of Applied Clinical Pediatrics》 2007-09
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Mechanisms of Stimulating Vagus Nerve on CD4~+ T Lymphocytes Activation in Experimental Arthritis Rats

WANG Jin-rong1a,LI Xiu-yun2,WANG Hong-wei2,FAN Hui1b,HAN Xiu-zhen1a,LIU Zhi-gang1a,SUN Yan1a,LI Hua-bing1a(1a.Department of Pediatrics ;1b.Department of Pathology,Shandong Provincial Hospital,Shandong University,Jinan 250021,China;2.Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)  
Objective To explore the effects of stimulating vagus nerve with pulse current on peripheral blood CD4+T lymphocyte of rats with collagen induced arthritis and its mechanism.Methods To duplicate model rats of experimental arthritis(EA)by intradermal injection of Ⅱtype collagen,divide the rats into 2 groups:vagus nerve stimulation(VNS)group and sham operated group.Rats in VNS group were stimulated at the left cervical vagus nerves for 30 minutes a day with constant square wave,pulse current with intra train of 16 Hz,pulse duration of 1.0 ms,train duration of 10 s,interstimulus interval of 1.5 min and intensities of 3.0 mA.Then flow cytometry and immunofluorescence methods were used to detect the activation of CD4+T lymphocytes(expressing CD71)and the expression of nicotinic acetylcholine receptors alpha 7(nAChRα7)and choline acetyltransferase(ChAT)in peripheral blood CD4+T lymphocytes.Results In VNS group,the expression of nAChRα7 and ChAT were significantly raised in CD4+ T cells at 1st weekend(Pa0.01);CD71+/CD4+ T cells in peripheral blood of EA rats were reduced significantly at the 2nd weekend(P0.01);the arthritis index of EA rats were stepped down markedly at the 3rd weekend(P0.01).Conclusion Vagus nerve stimulation can up-regulate the expression of nAChRα7 at the surface of CD4+T lymphocytes,activate the cholinergic system in immune system,which perform a local and constant immune regulation to reduce the inflammation at last.
【Fund】: 国家自然科学基金项目资助(30571739);; 山东省自然科学基金项目资助(Y2005C39)
【CateGory Index】: R593.2
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