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《Journal of Applied Clinical Pediatrics》 2007-17
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Expression of α-Smooth Muscle Actin in Rats with Tubulointerstitial Fibrosis

LI Nuan,YANG Da-sheng(Department of Pediatrics,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,China)  
Objective To observe the expression of α-smooth muscle actin(α-SMA)in rats with tubulointerstitial fibrosis and explore the possible mechanism of renal-protecting of α-SMA.Methods Sixty male Sprague-Dawley(SD)rats,aged 3 months,were randomly divided into 2 groups:control group(n=30)and model group(n=30).The renal tubulointerstitial fibrosis model was established by gavage with 150 mg/(kg·d)adenine solved by a solution of 20 g/L starch and the same volume starch was given to the rats from control group.At the 7th,12th,17th week,10 rats of every group were killed,the urinary protein,N-acetyl-β-D-glucosaminidase(NAG)in the urine and renal profile including BUN and serum Cr were examined.The histological changes of kidney were observed by light microscopy,and the expression of transforming growth factor-beta 1(TGF-β1)and α-SMA were examined by immunohistochemistry staining.Results At the 7th,12th and 17th week,the value of urinary protein,urinary NAG,serum BUN and Cr of rats from model group were higher than those of controls(Pa0.01).Renal interstitium injury was seen in model group rats at the 7th week,and became aggravating after the 7th week.The expression of α-SMA in rats from model group increased,and the highest in the 17th week.Conclusion The increase of α-SMA enhances the development of tubulointerstitial fibrosis.
【CateGory Index】: R726.9
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