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《Journal of Applied Clinical Pediatrics》 2009-02
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Effects of Taurin on Expressions of Bcl-2,Bax and Caspase-3 Protein in Brain of Newborn Rats with Intrauterine Growth Restriction

LIU Li1,CHEN Hui2,LIU Na1,LIU Jing2,JING Peng1,YANG Lian-xue1(1.Department of Anatomy and Histology,Capital Medical University,Beijing 100069,China;2.Department of Neonatology,Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University,Beijing 100026,China)  
Objective To explore the effect of Taurin on the expressions of Bcl-2,Bax and Caspase-3 protein in brain of newborn rats with intrauterine growth restriction(IUGR).Methods Fifteen pregnant rats were randomly divided into 3 groups:control group,IUGR model group,and taurin-treated group(n=5).Rats in IUGR model group and taurine-treated group were feed by 40% amount of the daily normal food of control group(10 g/d)since the first day after they were pregnant;meanwhile,the taurine-treated group were feed with Taurine 300 mg/(kg·d)since the 12th day after they were pregnant.Two newborn rats were chosen in every litter.The expressions of Bcl-2,Bax and Caspase-3 proteins in brain tissue of the newborn rats were detected with immunohistochemistry.The results were analyzed by SPSS 10.0 software.Results Compared with the control group,the number of Bcl-2 positive cells in IUGR model group was decreased,alterations in Bax and Caspase-3 were increased,and Bcl-2/Bax was lower,the differences were statistically significant(Pa0.05).Compared with IUGR model group,in taurine-treated group,the number of Bcl-2 positive cells was increased,alterations in Bax and Caspase-3 were decreased,and Bcl-2/Bax was higher,the differences were statistically significant(Pa0.05).In addition,the differences were also statistically significant between taurine-treated group and control group(Pa0.05).Conclusions Taurin can inhibit apoptosis of nerve cell and protect brain tissue of the newborn rats by regulating up the expression of Bcl-2 protein and regulating down that of Bax protein and Caspase-3 protein.
【Fund】: 首都医科大学基础-临床科研合作基金(2007JL53)
【CateGory Index】: R96
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