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《Journal of Applied Clinical Pediatrics》 2009-08
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Change of Cytochrome Oxidase Expression in Type Ⅱ Alveolar Epithelial Cell of Rats Exposed to Hyperoxia

PAN Rui,CHANG Li-wen,CHEN Yan(Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China)  
Objective To observe the expression of mitochondrial encoding cytochrome oxidase subunitⅠ(COXⅠ) and subunitⅡ(COXⅡ) in type Ⅱalveolar epithelial cell(AEC Ⅱ) of rats exposed to hyperoxia and explore the role of COXⅠand COXⅡ in hyperoxia-induced lung injury.Methods AECⅡ were gained by primary culture from 19-days fetal rats lung.After purified,AECⅡ were randomly assigned to hyperoxia group and air group.Hyperoxia group was flushed the flake with 950 mL/L O2 and 50 mL/L CO2 at 3 L/min for 10 min,then sealed.Both groups were in CO2 culture chamber(37 ℃,50 mL/L CO2).After 6,12 and 24 hours of exposure,AECⅡ were harvested and extracted for total RNA.COXⅠand COXⅡ mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).The results were analyzed by SPSS 12.0 softwore.Results Compared with air group,COXⅠmRNA expression in hyperoxia group increased significantly at 6 hours(t=3.832 P=0.019) and 12 hours(t=10.431 P=0),respectively,then decreased to the equivalent level in 24 hours(t=0.360 P=0.731).Compared with air group,COXⅡmRNA expression in hyperoxia group increased significantly at 6 hours(t=2.795 P=0.035),then decreased to the equivalent level at 12 hours(t=0.892 P=0.40) and 24 hours(t=2.018 P=0.071).Conclusions Exposure of hyperoxia up-regulate the expressions of COXⅠmRNA and COXⅡmRNA in AECⅡ,which may be a protective mechanism of hyperoxia-induced lung injury.
【Fund】: 国家自然科学基金项目资助(30672261)
【CateGory Index】: R725.6;R722.6
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1 HUANG Wan-Jie,XUE Xin-Dong,GUO Jing,YAO Li,FU Jian-Hua,QI Yong-Jun.Department of Pediatrics,Shengjing Hospital,China Medical University,Shenyang 110004,China;Early clinical presentations and MRI characteristics in newborns with cerebral infarction[J];Chinese Journal of Contemporary Pediatrics;2009-02
2 WANG Wei,QIAN Li-Ling,LIU Hai-Pei,SUN Bo Laboratory of Pediatric Respiratory and Critical Care Medicine,Children's Hospital andthe Institutes of Biomedical Sciences,Fudan University,Shanghai 201102,China;Engraftment of bone marrow stromal cells in lipopolysaccharide-injured mouse lungs[J];Chinese Journal of Contemporary Pediatrics;2009-05
3 WANG Qing-Hong, YANG Yu-Jia, CHEN Chong-Feng, YAO Yue, LI Meng. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China;Protective effects of delayed multiple course hyperbaric oxygen treatment against hypoxic-ischemic brain damage in neonatal rats[J];Chinese Journal of Contemporary Pediatrics;2009-06
4 DUAN Jiang1,2, CHEN Hong-Qing2, LI Ran2, YIN Xiao-Juan2, FENG Zhi-Chun21Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China, 2Bayi Children's Hospital, General Hospital, Beijing Military Area Command, Beijing 100700, China;Influence of TGF-β1 siRNA mediated by lentivirus vector on hyperoxia-induced lung injury in neonatal mice[J];Journal of the Fourth Military Medical University;2009-09
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8 HUANG Yu-ge1,FENG Zhi-chun2,YU Yan-liang1,XIAO Fang-fang1(1.Department of Pediatrics,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,China;2.Bayi Children's Hospital,The Military General Hospital of Beijing PLA,Beijing 100700,China);Protective effects of MG-132 on p38 signaling pathway and cell apoptosis in lung injury induced by hyperoxia[J];Journal of Shanghai Jiaotong University(Medical Science);2009-08
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