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《Journal of Applied Clinical Pediatrics》 2009-14
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Effects of Various Dosage and Multiple Course of Dexamethasone on Expression of WNT Signal Transduction Pathway in Lung of Premature Rats

WANG Quan,ZHAO De-yu(Department of Respiratory Medicine,Nanjing Children's Hospital Affiliated to Nanjing Medical University,Nanjing 210008,Jiangsu Pro-vince,China)  
Objective To explore the effects of various dosage and multiple course of Dexamethasone(DEX) on the expressions of WNTs,β-catenin and glycogen synthase kmase-3β(GSK-3β) genes in the lung of premature rats on the 19th day of embryo.Methods Twelve pregnant SD rats were divided into 3 groups randomly:small dose DEX group,large dose DEX group and control group with 4 rats in each group.The rats in control group were injected with saline 9 g/L;rats in small dose DEX group were injected with DEX 0.4 mg/(kg·d),and the rats in large dose DEX group were injected with DEX 0.8 mg/(kg·d) after DEX was diluted to 0.5 mL with saline.On the 19th day of gestation,fetuses were surgically taken out.The reverse transcription polymerase chain reaction PCR method was used to detect expressions of WNT7b,WNT5a,WNT2,GSK-3β and β-catenin genes mRNA.Results The expressions of WNT7b(0.55±0.19,0.64±0.54)and β-catenin(2.03±0.58,2.40±0.89)genes mRNA in small dose DEX group and large dose DEX group were significantly higher than those of control group(WNT7b:0.18±0.10,β-catenin:1.77±0.54)(Pa0.01),while the expressions of WNT5a(0.57±0.26,1.13±0.53)and GSK-3β(1.04±0.46,1.09±0.56)were lower than those of control group(WNT5a:1.48±0.70,GSK-3β:2.22±1.02)(P0.05,0.01),and the expression of WNT2 showed no difference between DEX groups and control group.Conclusions Antenatal DEX usage can change the expressions of WNT7b,WNT5a,β-catenin and GSK-3β genes mRNA,and these changes may result in the abnormal WNT7b signal transduction pathway and may lead to the paramorphia during the pregnancy.
【CateGory Index】: R722.6
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