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《Acta Academiae Medicinae Suzhou》 2001-04
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The Utilization of Ultrasonication in Mutagenic Breeding of Protoplast

LU Ming,QI Ru-feng,XU Pei-jun,et al (Department of Microbiology,Suzhou Unirersity,Suzhou 215007,China)  
Objective Improve the production of a kind of spiramycin-producing strain-S.ambifaciens pinnert-Sindico.Methods Ultrasonication was used to disintergrate the cell wall of the resource strain,in this way,we got the protoplast.After growing in the regeneration medium,the test-strains improved their production in great area.Results In the strains of the first selection,the 38.3 % of them improved the production after fermentation.The increasing rate was from 1.62% to 11.92 %.Then the second selection was given to those high-production strains in the first selection,after the fermentation,the production is still at high level.The increasing rate was from 5.48% 11.92 %,the increasing rate was 8.73% in average.Conclusion The ultrasonication used in getting protoplast is operational,cheap,and the results are less affected by various factors.The method is suitable for the mutagenesis breeding in domestic factories.
【CateGory Index】: R915
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