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《Journal of Shenyang Pharmaceutical University》 2002-02
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Measurement of the four tetracycline residues in the milk by high-performance liquid chromatography mass spectrometry

JIA Wei, SUN Lu, SHI Xiang guo, CHEN Xiao yan, ZHONG Da fang (Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, China)  
Objective Developing a method for determination of residues of tetracyclines, oxytetracycline, chlortetracycline and metacycline in the milk. Method The tetracyclines were extracted from the milk using solid phase extraction and were determined by liquid chromatography electrospray mass spectrometry with the mobile phase of acetonitrile water formic acid (37 5∶62 5∶1 2, V/V) . Result The detection limits were 0 05 μg/mL for tetracyclines, oxytetracycline and metacycline, and 0 1 μg/mL for chlortetracycline, respectively. The calibration curves are linear from 0 1 to 5 0 μg/mL for tetracyclines, oxytetracycline and metacycline, and 0 2 to 10 0 μg/mL for chlortetracycline. Conclusion This method was specific, sensitive, and suitable for determination of the residual tetracyclines in the milk or other biological samples. The pathways of mass fragmentation of tetracyclines were also interpreted.
【Fund】: 国家自然科学基金资助项目 ( 39930 1 80 )
【CateGory Index】: R155.5
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