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《Journal of Shenyang Pharmaceutical University》 2004-02
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Determination of the contents of chlorogenic acid and caffeic acid in the compound Daqingye Injection by RP-HPLC

YUAN Bo, LI Lei, LI Fa-mei(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)  
Objective To describe a reversed-phase HPLC method for the determination of the contents of chlorogenic acid and caffeic acid in the compound Daqingye Injection.Methods The samples were separated on a C8 column(5 μm,4.6 mm×200 mm) with the mobile phase of methanol-phosphate solution(φ=0.2%) pH 2.8 (V∶V=80∶20).The flow rate was 0.8 mL·min-1,and the detection was set at 323 nm.Results The average recovery of the chlorogenic acid was 100.0%,RSD=1.1%;the average recovery of Caffeic acid was 100.0%,RSD=1.9%.Conclusion The method can be used for the quality control of the compound Daqingye Injection.
【CateGory Index】: R286.0
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